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INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY logo

Volume 74, Issue 4

sp. nov., a moderately halophilic bacterium from Urmia Lake, Iran No Access

Abstract

A novel moderately halophilic, Gram-stain-negative and facultatively anaerobic bacterium, designated as strain TBZ242, was isolated from water of Urmia Lake in the Azerbaijan region of Iran. The cells were found to be rod-shaped and motile by a single polar flagellum, producing circular and yellowish colonies. The strain could grow in the presence of 0.5–10 % (w/v) NaCl (optimum, 2.5–5 %). The temperature and pH ranges for growth were 15–45 °C (optimum 30 °C) and pH 7.0–11.0 (optimum pH 8.0) on marine agar. The 16S rRNA gene sequence analysis revealed that strain TBZ242 belonged to the genus , showing the highest similarities to DG893 (98.8 %), F01 (98.8 %), R9SW1 (98.5 %), PJ-16 (98.4 %), W62 (98.0 %) and JB2H27 (98.0 %). The 16S rRNA and core-genome phylogenetic trees showed that strain TBZ242 formed a distinct branch, closely related to a subclade accommodating , , , , , and , within the genus . Average nucleotide identity and digital DNA–DNA hybridization values between strain TBZ242 and the type strains of the related species of were ≤85.0 and 28.6 %, respectively, confirming that strain TBZ242 represents a distinct species. The major cellular fatty acids of strain TBZ242 were C and C 7/C ω6 and the quinone was ubiquinone Q-9. The genomic DNA G+C content of strain TBZ242 is 57.2 mol%. Based on phenotypic, chemotaxonomic and genomic data, strain TBZ242 represents a novel species within the genus , for which the name sp. nov. is proposed. The type strain is TBZ242 (= CECT 30649 = IBRC-M 11466). Genomic fragment recruitment analysis showed that this species prefers aquatic saline environments with intermediate salinities, being detected on metagenomic databases of Lake Meyghan (Iran) with 5 and 18 % salinity, respectively.

  • Received:
  • Accepted:
  • Published Online:
Keyword(s): comparative genomics , halophilic bacteria , Marinobacter , new taxa , taxonomy and Urmia Lake
Funding
This study was supported by the:
  • Consejería de Economía, Innovación, Ciencia y Empleo, Junta de Andalucía (Award BIO-213)
    • Principle Award Recipient: VentosaAntonio
  • Consejería de Economía, Conocimiento, Empresas y Universidad, Junta de Andalucía (Award P20_01066)
    • Principle Award Recipient: VentosaAntonio
  • MICIN/AEI/10.13039/501100011033 (Award PID2020-118136GB-I00)
    • Principle Award Recipient: VentosaAntonio
  • Tabriz University of Medical Sciences (Award IR.TBZMED.VCR.REC.1400.320)
    • Principle Award Recipient: Saeid HejaziMohammad
© 2024 The Authors
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Marinobacter azerbaijanicus sp. nov., a moderately halophilic bacterium from Urmia Lake, Iran
Int J Syst Evol Microbiol 74, 006308 (2024); https://doi.org/10.1099/ijsem.0.006308
/content/journal/ijsem/10.1099/ijsem.0.006308
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