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INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY logo International Journal of Systematic and Evolutionary Microbiology

Volume 74, Issue 3

gen. nov., sp. nov., a novel taxon within the family isolated from sludge No Access

Abstract

A Gram-stain-positive bacterium, designated YN-L-19, was isolated from a sludge sample collected from a pesticide-manufacturing plant. Cells of YN-L-19 were strictly aerobic, non-spore-forming, non-motile and ovoid-shaped. Colonies were small, smooth and yellow. Growth occurred at 10–37 °C (optimum, 30 °C), pH 5.0–9.0 (optimum, 7.0) and 0–3.0 % (w/v) NaCl (optimum 0.5 %). Phylogenetic analysis based on genome and 16S rRNA gene sequences indicated that YN-L-19 was affiliated to the family and most closely related to , , and . The major cellular fatty acids of YN-L-19 were anteiso-C, anteiso-C, iso-C and C. The predominant menaquinone was MK-7. The major polar lipids were diphosphatidylglycerol, phosphatidylglycerol, glycolipid and one unidentified lipid. The average amino acid identity values between strain YN-L-19 and the related strains were 57.9–61.9 %, which were below the genus boundary (70 %). On the basis of the evidence presented in this study, strain YN-L-19 represents a novel species of a new genus in the family , for which the name gen. nov., sp. nov. (type strain YN-L-19=CCTCC AB 2022401= KCTC 49935) is proposed.

  • Received:
  • Accepted:
  • Published Online:
Keyword(s): Actinomycetota , Microbacteriaceae , Ruicaihuangia caeni and taxonomy
Funding
This study was supported by the:
  • Science and Technology Program of Jiangsu Province (Award BM2022019)
    • Principle Award Recipient: HeJian
© 2024 The Authors
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/content/journal/ijsem/10.1099/ijsem.0.006302
2024-03-26
2025-04-25
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Ruicaihuangia caeni gen. nov., sp. nov., a novel taxon within the family Microbacteriaceae isolated from sludge
Int J Syst Evol Microbiol 74, 006302 (2024); https://doi.org/10.1099/ijsem.0.006302
/content/journal/ijsem/10.1099/ijsem.0.006302
/content/journal/ijsem/10.1099/ijsem.0.006302
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