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Volume 74, Issue 4

sp. nov., a uranium-resistant bacterium isolated from uranium-contaminated soil No Access

Abstract

A novel Gram-positive strain, B1, was isolated from uranium-contaminated soil. The strain was aerobic, rod-shaped, spore-forming, and motile. The strain was able to grow at 20−45 °C, at pH 6.0–9.0, and in the presence of 0–3 % (w/v) NaCl. The complete genome size of the novel strain was 3 853 322 bp. The genomic DNA G+C content was 45.5 mol%. Phylogenetic analysis based on the 16S rRNA gene sequence showed that strain B1 has the highest similarity to CB4 (96. 71 %). However, the novel strain showed an average nucleotide identity value of 89.02 % and a digital DNA–DNA hybridization value of 37.40 % with strain CB4 based on the genome sequences. The major fatty acids were iso-C and C. The predominate respiratory quinone was MK7. Diphosphatidylglycerol, phosphatidylmethylethanolamine, phosphatidylethanolamine, phosphatidylglycerol, unidentified phospholipids, an unidentified aminolipid and an unidentified lipid were identified as the major polar lipids. The phylogenetic, phenotypic, and chemotaxonomic analyses showed that strain B1 represents a novel species of the genus , for which the name sp. nov. is proposed. The type strain is B1 (=GDMCC 1.4080=JCM 36228). Experiments have shown that strain B1 demonstrates uranium tolerance.

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  • Accepted:
  • Published Online:
Keyword(s): Aneurinibacillus and uranium tolerance
Funding
This study was supported by the:
  • Project of Fund for Stable Support to Agricultural Sci-Tech Renovation sponsored by Xinjiang Academy of Agricultural Science (Award xjnkywdzc-2023005-6)
    • Principle Award Recipient: LijuanZhang
  • the project of renovation capacity building for the young sci-tech talents sponsored by Xinjiang Academy of Agricultural Science (Award xjnkq-2023013)
    • Principle Award Recipient: NingWang
  • the Natural Science Foundation of Xinjiang Uygur Autonomous Region (Award 2022D01A266)
    • Principle Award Recipient: LijuanZhang
  • the National Key Research and Development Program of China (Award 2020YFC1806603)
    • Principle Award Recipient: WeiWang
© 2024 The Authors
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Aneurinibacillus uraniidurans sp. nov., a uranium-resistant bacterium isolated from uranium-contaminated soil
Int J Syst Evol Microbiol 74, 006297 (2024); https://doi.org/10.1099/ijsem.0.006297
/content/journal/ijsem/10.1099/ijsem.0.006297
/content/journal/ijsem/10.1099/ijsem.0.006297
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