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INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY logo

Volume 74, Issue 3

gen. nov., sp. nov., isolated from river sediment represents a novel genus of the family No Access

Abstract

A Gram-stain-negative bacterium, designated as R-40, was isolated from sediment of the Mulong river in Mianyang city, Sichuan province, PR China. The cells of strain R-40 were aerobic non-motile and formed translucent white colonies on R2A agar. Growth occurred at 15–37 °C (optimum 30 °C), pH 5.0–9.0 (optimum 7.0) and salinities of 0–3.0 % (w/v, optimum 0 %). R-40 showed 95.2–96.6 % 16S rRNA gene sequence similarities with the type strains of species of the genera , , , and in the family . The results of phylogenetic analysis based on genome sequences indicated that the strain was clustered with type strains of species of the genera and in the family but formed a distinct lineage. The average nucleotide identity (ANI), digital DNA–DNA hybridization (dDDH) and average amino acid identity (AAI) values between R-40 and type strains of species of the genera , , , and ranged from 69.3 to 74.1 %, from 18.2 to 21.4 % and from 60.1 to 67.4 %, respectively. The major cellular fatty acids were C, C cyclo and summed feature 3 (Cω7 and/or Cω6). The major quinone was ubiquinone-8 (Q-8). The polar lipid profile consisted of diphosphatidylglycerol, phosphatidylethanolamine, phosphatidylglycerol, phospholipid and small amounts of glycophospholipids. The genome size of R-40 was 5.1 Mbp with 54.0 % DNA G+C content. On the basis of the evidence presented in this study, strain R-40 represents a novel species of a novel genus in the family , for which the name gen. nov., sp. nov. (type strain R-40=MCCC 1K08818=KCTC 8137) is proposed.

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  • Accepted:
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Keyword(s): 16S rRNA gene , Keguizhuia sedimentigen. nov., sp. Nov , overall genome relatedness index , polyphasic taxonomy and sludge
Funding
This study was supported by the:
  • National Natural Science Foundation of China (Award No. 32170128)
    • Principle Award Recipient: ShiyuZhao
© 2024 The Authors
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2024-04-28
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Keguizhuia sedimenti gen. nov., sp. nov., isolated from river sediment represents a novel genus of the family Oxalobacteraceae
Int J Syst Evol Microbiol 74, 006289 (2024); https://doi.org/10.1099/ijsem.0.006289
/content/journal/ijsem/10.1099/ijsem.0.006289
/content/journal/ijsem/10.1099/ijsem.0.006289
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