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Volume 74, Issue 2

sp. nov., a novel thermophilic cellulose- and starch-degrading member of the from a geothermal aquifer in Kazakhstan Open Access

Abstract

A thermophilic, aerobic and heterotrophic filamentous bacterium, designated strain ZKZ2, was isolated from a pipeline producing hydrothermal water originating from a >2.3 km deep subsurface geothermal source in Zharkent, Almaty region, Kazakhstan. The isolate was Gram-stain-positive, non-motile, heat-resistant and capable of producing a variety of extracellular hydrolases. Growth occurred at temperatures between 55 and 75 °C, with an optimum around 70 °C, and at pH values between 5.5 and 9.0, with an optimum at pH 7.0–7.5 with the formation of aerial mycelia; endospores were produced along the aerial mycelium. The isolate was able to utilize the following substrates for growth: glycerol, -arabinose, ribose, -xylose, -glucose, -fructose, -mannose, rhamnose, -mannitol, methyl--glucopyranoside, aesculin, salicin, cellobiose, maltose, melibiose, sucrose, trehalose, melezitose, raffinose, starch, turanose and 5-keto-gluconate. Furthermore, it was able to hydrolyse carboxymethylcellulose, starch, skimmed milk, Tween 60 and Tween 80. The major cellular fatty acids were iso-C, iso-C, iso-C and C. Our 16S rRNA gene sequence analysis placed ZKZ2 within the genus , family , with the highest similarity to the type species JIR-001 (99.18 % sequence identity). Our draft genome sequence analysis revealed a genome size of 3.3 Mbp with a G+C value of 52.5 mol%. The orthologous average nucleotide identity value as compared to that of its closest relative, JIR-001, was 90.23 %, with an DNA–DNA hybridization value of 40.7 %, indicating that ZKZ2 represents a separate genome species. Based on the phenotypic and genome sequence differences from the other two species, we propose that strain ZKZ2 represents a novel species, for which we propose the name sp. nov. The type strain is ZKZ2 (=CECT 30708=KCTC 43421).

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Keyword(s): geothermal spring in Kazakhstan , Polycladomyces zharkentensis and thermophilic filamentous bacterium
Funding
This study was supported by the:
  • CPEA-LT-2017/10061
    • Principle Award Recipient: Nils-KåreBirkeland
  • AP14871683
    • Principle Award Recipient: AidaKistaubayeva
© 2024 The Authors
  • This is an open-access article distributed under the terms of the Creative Commons Attribution License. This article was made open access via a Publish and Read agreement between the Microbiology Society and the corresponding author’s institution.
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2024-02-26
2024-04-28
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Polycladomyces zharkentensis sp. nov., a novel thermophilic cellulose- and starch-degrading member of the Bacillota from a geothermal aquifer in Kazakhstan
Int J Syst Evol Microbiol 74, 006269 (2024); https://doi.org/10.1099/ijsem.0.006269
/content/journal/ijsem/10.1099/ijsem.0.006269
/content/journal/ijsem/10.1099/ijsem.0.006269
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