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INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY logo

Volume 74, Issue 2

Three novel species of the genus , sp. nov., sp. nov. and sp. nov., isolated from activated sludge No Access

Abstract

Three Gram-stain-negative, rod-shaped, non-spore-forming bacteria were isolated from activated sludge samples. The results of phylogenetic analysis based on the 16S rRNA gene sequences indicated that the three strains, designated HXWNR29, HXWNR69 and HXWNR70, had the highest sequence similarity to the type strains NJ-26, KSM-R2A30 and KYPY10 with similarities of 97.66 %, 98.66 and 98.14 %, respectively. The draft genomes of these three strains were 2.93 Mbp (HXWNR29), 2.69 Mbp (HXWNR69) and 2.65 Mbp (HXWNR70) long with DNA G+C contents of 31.84 %, 32.83 % and 34.66 %, respectively. These genomes contained many genes responsible for carbohydrate degradation and antibiotic resistance. The major fatty acids (>5 %) included iso-C, iso-C 3-OH and iso-C 3-OH. The major menaquinone was MK-6 for all the three strains. The average nucleotide identity (ANI; 72.7–88.5 %) and digital DNA–DNA hybridization (dDDH; 19.6–35.3 %) results further indicated that these three strains represented three novel species within the genus , for which the names sp. nov. (type strain HXWNR29 = KCTC 92446 = CGMCC 1.61821), sp. nov. (type strain HXWNR69 = KCTC 92468 = CGMCC 1.61442) and sp. nov. (type strain HXWNR70 = KCTC 92447 = CGMCC 1.61443) are proposed.

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  • Accepted:
  • Published Online:
Keyword(s): Flavobacterium , novel species and Polyphasic taxonomy
Funding
This study was supported by the:
  • National Natural Science Foundation of China (Award 32070030)
    • Principle Award Recipient: Yue-zhongLi
  • National Natural Science Foundation of China (Award 32300090, 32261143736)
    • Principle Award Recipient: JingjingWang
  • Ministry of Science and Technology of the People's Republic of China (Award 2022FY101100)
    • Principle Award Recipient: JingjingWang
© 2024 The Authors
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2024-05-11
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Three novel species of the genus Flavobacterium, Flavobacterium odoriferum sp. nov., Flavobacterium fragile sp. nov. and Flavobacterium luminosum sp. nov., isolated from activated sludge
Int J Syst Evol Microbiol 74, 006258 (2024); https://doi.org/10.1099/ijsem.0.006258
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