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Volume 74, Issue 1

sp. nov. and sp. nov., two members of the phylum isolated from the wastewater treatment system of a pharmaceutical factory No Access

Abstract

Five strains of two novel species were isolated from the wastewater treatment systems of a pharmaceutical factory located in Zhejiang province, PR China. Strains ZM22 and Y6 were identified as belonging to a potential novel species of the genus , whereas strains ZM23, ZM24 and ZM25 were identified as belonging to a novel species of the genus . These strains were characterized by polyphasic approaches including 16S rRNA gene analysis, multi-locus sequence analysis, average nucleotide identity (ANI), DNA–DNA hybridization (DDH), physiological and biochemical tests, as well as chemotaxonomic analysis. Genome-based phylogenetic analysis further confirmed that strains ZM22 and Y6 form a distinct clade closely related to ATCC 11996 and DSM 17888. Strains ZM23, ZM24 and ZM25 were grouped as a separate clade closely related to DSM 14399 and LAM1902. The orthoANI and DDH results indicated that strains ZM22 and Y6 belong to the same species. In addition, genomic DNA fingerprinting demonstrated that these strains do not originate from a single clone. The same results were observed for strains ZM23, ZM24 and ZM25. Strains ZM22 and Y6 were resistant to multiple antibiotics, whereas strains ZM23, ZM24 and ZM25 were able to degrade an emerging pollutant, triclosan. The phylogenetic, physiological and biochemical characteristics, as well as chemotaxonomy, allowed these strains to be distinguished from their genus, and we therefore propose the names sp. nov. (type strain ZM22=MCCC 1K08496=KCTC 82561) and sp. nov. (type strain ZM23=MCCC 1K08497=JCM 36056), respectively.

  • Received:
  • Accepted:
  • Published Online:
Keyword(s): activated sludge , Comamonas , novel species , polyphasic taxonomy , Pseudomonas and triclosan
Funding
This study was supported by the:
  • Zhejiang Xinmiao Talents Program (Award 2023R401025)
    • Principle Award Recipient: JiayuHan
  • Innovative Research Group Project of the National Natural Science Foundation of China (Award 41721001)
    • Principle Award Recipient: ZhenmeiLu
  • National Natural Science Foundation of China (Award 32370117)
    • Principle Award Recipient: ZhenmeiLu
  • the Key Research and Development Program of Zhejiang Province (Award 2021C03168)
    • Principle Award Recipient: ZhenmeiLu
© 2024 The Authors
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2024-01-08
2024-05-17
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Comamonas resistens sp. nov. and Pseudomonas triclosanedens sp. nov., two members of the phylum Pseudomonadota isolated from the wastewater treatment system of a pharmaceutical factory
Int J Syst Evol Microbiol 74, 006222 (2024); https://doi.org/10.1099/ijsem.0.006222
/content/journal/ijsem/10.1099/ijsem.0.006222
/content/journal/ijsem/10.1099/ijsem.0.006222
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