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Volume 73, Issue 12

sp. nov., isolated from cow faeces No Access

Abstract

An aerobic, Gram-stain-positive, rod-shaped, endospore-forming bacterial strain, designated BB3-R1, was isolated from cow faeces sampled in Daejeon, Republic of Korea. Growth was observed at 25–45 °C (optimum, 35–40 °C) and pH 7.0–9.0 (optimum, pH 8.0), with up to 3 % (w/v) NaCl (optimum, 0 % NaCl). analysis of 16S rRNA gene sequences revealed the highest sequence similarity of strain BB3-R1 to NRRL NRS-818 (98.8 %) followed by JCM 15085 (97.5 %). According to 16S rRNA gene and whole-genome based phylogenetic trees, strain BB3-R1 clustered with FJAT-54423 and NRRL NRS-818. OrthoANI and dDDH values of strain BB3-R1 with the closely related strains were lower than 77.5 and 26.8 %, respectively. The major menaquinones and polar lipids of the strain were MK-7 and phosphatidylmonomethylethanolamine, diphosphatidylglycerol, phosphatidylglycerol and phosphatidylethanolamine, respectively. The major fatty acids (>10 %) were C iso, C iso, C anteiso and C  7 alcohol. The cell-wall peptidoglycan contained cross-linked -diaminopimelic acid (type A1 gamma). The phenotypic, chemotaxonomic and genotypic data obtained in this study showed that the strain represents a novel species of the genus , for which the name sp. nov. (type strain BB3-R1=KACC 22663=NBRC 115962) is proposed.

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Keyword(s): Brevibacillus ruminantium sp. nov. , cow faeces , novel species and taxonomy
© 2023 The Authors
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2023-12-14
2024-05-04
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Brevibacillus ruminantium sp. nov., isolated from cow faeces
Int J Syst Evol Microbiol 73, 006204 (2023); https://doi.org/10.1099/ijsem.0.006204
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