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Volume 73, Issue 12

sp. nov., a chemolithoautotrophic sulphur-oxidizing and nitrogen-fixing bacterium isolated from freshwater No Access

Abstract

A sulphur-oxidizing and nitrogen-fixing bacterium, designated strain LS2, was isolated from freshwater collected from the Pearl River in Guangzhou, PR China. The strain was an obligate chemolithoautotroph, utilizing reduced sulphur compounds (sulphide, sulphite, elemental sulphur, thiosulphate and tetrathionate) as energy sources and electron donors. Diazotrophic growth of strain LS2 was observed at 15–40 °C, pH 5–9, with a NaCl concentration range of 0–0.68 mol l and with oxygen content higher than 21 %. The major cellular fatty acids were summed feature 8 (comprising C ω7 and/or C ω6) and C. The DNA G+C content of the complete genome sequence was 60.7 mol%. Phylogenetic analysis based on the 16S rRNA gene sequence showed that strain LS2 formed a lineage within the family , showing gene sequence identity of 96.8 % with its closest relative c2. The genome of strain LS2 contains multiple genes encoding sulphur-oxidizing enzymes that catalyse the oxidation of reduced sulphur compounds and an complex encoding enzymes for nitrogen fixation. In addition, the genome contains genes encoding -type cytochrome c oxidase, -type cytochrome c oxidase, -type quinol oxidase and cytochrome o oxidase, which enable the survival strain LS2 under oxic and microaerophilic conditions. On the basis of phenotypic, genotypic and phylogenetic data, strain LS2 is considered to represent a novel species of the genus , for which the name sp. nov. is proposed. The type strain is LS2 (=GDMCC 1.4095=JCM 39442).

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  • Accepted:
  • Published Online:
Keyword(s): Halothiobacillaceae , Halothiobacillus diazotrophicus , nitrogen-fixing bacterium and sulphur-oxidizing bacterium
Funding
This study was supported by the:
  • Fundamental Research Funds for the Central Universities (Award 2022ZYGXZR040)
    • Principle Award Recipient: JianfeiLuo
  • Basic and Applied Basic Research Foundation of Guangdong Province (Award 2021A1515010565)
    • Principle Award Recipient: JianfeiLuo
  • National Natural Science Foundation of China (Award 91951118)
    • Principle Award Recipient: JianfeiLuo
  • National Natural Science Foundation of China (Award 41977034)
    • Principle Award Recipient: WeitieLin
© 2023 The Authors
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2023-12-12
2024-05-08
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Halothiobacillus diazotrophicus sp. nov., a chemolithoautotrophic sulphur-oxidizing and nitrogen-fixing bacterium isolated from freshwater
Int J Syst Evol Microbiol 73, 006202 (2023); https://doi.org/10.1099/ijsem.0.006202
/content/journal/ijsem/10.1099/ijsem.0.006202
/content/journal/ijsem/10.1099/ijsem.0.006202
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