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INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY logo

Volume 73, Issue 12

sp. nov., sp. nov., sp. nov. and sp. nov., isolated from in, and around, a rainbow trout farm No Access

Abstract

During a large-scale bacterial culturing effort of biofilms in the vicinity of a rainbow trout aquaculture facility in Idaho, USA, 10 isolates were identified as having pathogen-inhibiting activity and were characterized further. These isolates were shown to be Gram-negative, rod-shaped bacteria belonging to the genus . Whole-genome comparisons and multi-locus sequence analysis using four housekeeping genes (16S rRNA, , and ) showed that these 10 isolates clustered into four distinct species groups. These comparisons also indicated that these isolates were below the established species cutoffs for the genus . Further phenotypic characterization using API 20NE, API ZYM and Biolog GENIII assays and chemotaxonomic analysis of cellular fatty acids were carried out. Based on the genomic, physiological and chemotaxonomic properties of these isolates, we concluded that these strains composed four novel species of the genus . The proposed names are as follows: sp. nov. consisting of strains ID233, ID386 and ID387 with ID386 (=DSM 114641=ATCC TSD-305) as the type strain; sp. nov. consisting of strains ID291, ID609 and ID1025 with ID291 (=DSM 114640=ATCC TSD-303) as the type strain; sp. nov. consisting of strains ID357 and ID1048 with ID357 (=DSM 114609=ATCC TSD-304) as the type strain; and sp. nov. consisting of strains ID656 and ID681 with ID656 (=DSM 114610=ATCC TSD-306) as the type strain.

  • Received:
  • Accepted:
  • Published Online:
Keyword(s): aquaculture , biofilm , freshwater , Pseudomonas and rainbow trout
Funding
This study was supported by the:
  • U.S. Department of Agriculture (Award 8082-32000-007-000-D)
    • Principle Award Recipient: GrafJoerg
© 2023 The Authors
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/content/journal/ijsem/10.1099/ijsem.0.006201
2023-12-18
2024-05-08
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Pseudomonas aphyarum sp. nov., Pseudomonas fontis sp. nov., Pseudomonas idahonensis sp. nov. and Pseudomonas rubra sp. nov., isolated from in, and around, a rainbow trout farm
Int J Syst Evol Microbiol 73, 006201 (2023); https://doi.org/10.1099/ijsem.0.006201
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