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Volume 73, Issue 12

sp. nov., a halophilic nitrite-reducing bacterium, isolated from intertidal surface sediments No Access

Abstract

A novel Gram-stain-negative strain, designated S37H4, was isolated from an intertidal surface sediment sample collected from Zhanjiang City, Guangdong province, south PR China. Cells of the strain were aerobic, non-flagellated, long rod-shaped and motile by gliding. S37H4 could grow at 4–40 °C, pH 7.0–8.5 and in 2.0–15.0 % NaCl, with optimal growth at 25–30 °C, pH 7.5 and 9.0 % NaCl, respectively. S37H4 was capable of nitrite removal under high-salt conditions, and there were three denitrification genes, , and in its genome. The results of phylogenetic analyses based on the 16S rRNA gene and genome sequences indicated that S37H4 represented a member of the genus and formed a subclade with JLT2000. S37H4 showed the highest 16S rRNA sequence similarity to JLT2000 (98.3 %) and less than 97.0 % similarity with other type strains of species of the genus . The average nucleotide identity (ANI) and digital DNA–DNA hybridization (dDDH) values between S37H4 and the reference type strains of species of the genus were 70.7–74.3 % and 18.2–19.2 %, respectively. The major fatty acids of S37H4 were iso-C, iso-CG, iso-C 3-OH and summed feature 3 (Cω6 and/or Cω7). The major respiratory quinone of this novel strain was MK-7, and the predominant polar lipids were identified as phosphatidylethanolamine, an unidentified aminolipid, an unidentified phospholipid and three unidentified lipids. The results of analyses of phylogenetic, genomic, physiological and biochemical characteristics indicated that S37H4 represented a novel species of the genus , for which the name sp. nov. is proposed. The type strain is S37H4 (= GDMCC 1.1866 = KACC 21922).

  • Received:
  • Accepted:
  • Published Online:
Keyword(s): halophilic bacterium , Marivirga aurantiaca , nitrite removal , novel species and Polyphasic taxonomy
Funding
This study was supported by the:
  • Guangdong Special Support Program (Award 2021JC06N628)
    • Principle Award Recipient: HonghuiZhu
  • GDAS’ Project of Science and Technology Development (Award 2022GDASZH-2022010201)
    • Principle Award Recipient: YulianZhang
  • National Natural Science Foundation of China (Award 32200086)
    • Principle Award Recipient: MingxiaZhang
  • National Natural Science Foundation of China (Award 32070115)
    • Principle Award Recipient: MingxiaZhang
© 2023 The Authors
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/content/journal/ijsem/10.1099/ijsem.0.006195
2023-12-11
2024-05-08
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Marivirga aurantiaca sp. nov., a halophilic nitrite-reducing bacterium, isolated from intertidal surface sediments
Int J Syst Evol Microbiol 73, 006195 (2023); https://doi.org/10.1099/ijsem.0.006195
/content/journal/ijsem/10.1099/ijsem.0.006195
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