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Volume 73, Issue 12

sp. nov., isolated from tidal flat sediment No Access

Abstract

Two novel strains, SA3-5 and SA3-6, were isolated from a tidal flat (getbol) in the Republic of Korea. Strains SA3-5 and SA3-6 were subjected to polyphasic characterization to determine their taxonomic affiliations. Cells were Gram-stain-negative, aerobic, rod-shaped and motile by using peritrichous flagella. Based on their 16S rRNA gene sequences, strains SA3-5 and SA3-6 exhibited a high degree of similarity (100 %) and were classified within the genus . Furthermore, the closest related species to SA3-5 and SA3-6 were MS-3 (98.3 %). The ranges of average nucleotide identity and digital DNA–DNA hybridization values between SA3-5 and closely related species were 75.9–89.1% and 21.3–38.7%, respectively, both of which being below the thresholds for delineating novel strains. Strain SA3-5 and SA3-6 contained C 6 and/or C 7 (summed feature 3), C and C 6 and/or C 7 (summed feature 8) as the major fatty acids. The predominant respiratory quinone was Q-9. The DNA G+C content of strain SA3-5 was 62.5 mol%. Based on their combined phenotypic, chemotaxonomic and phylogenetic characterisitics, strains SA3-5 and SA3-6 represent a novel species of the genus for which the name sp. nov. is proposed. The type strain is SA3-5 (=KCTC 92395=JCM 35697).

  • Received:
  • Accepted:
  • Published Online:
Keyword(s): Getbol , Pseudomonas , taxonomy and tidal flat
Funding
This study was supported by the:
  • Korea Research Institute of Bioscience and Biotechnology (Award KGM5232322)
    • Principle Award Recipient: Jung-SookLee
© 2023 The Authors
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2023-12-04
2024-05-08
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Pseudomonas aestuarii sp. nov., isolated from tidal flat sediment
Int J Syst Evol Microbiol 73, 006190 (2023); https://doi.org/10.1099/ijsem.0.006190
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