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Volume 73, Issue 11

sp. nov., isolated from human blood Open Access

Abstract

An obligate anaerobic, Gram-negative, rod-shaped and non-spore-forming bacterium, designated as strain GYB001, was isolated from the blood of a patient with a sigmoid colon perforation. Taxonomic characterization of the novel isolate was performed using a polyphasic approach. A phylogenetic analysis based on 16S rRNA gene and whole genome sequences revealed that GYB001 represented a member of the genus , in the family . The closest species, based on 16S rRNA sequence, was DSM 23371 with 97.4 % similarity. Average nucleotide identity and digital DNA–DNA hybridization values between strain GYB001 and DSM 23371 were 86.7 and 28.7% and between GYB001 and JCM 18682 were 86.6 and 27.7 %, respectively. The genome was 6.57 Mbp long with 43.3 mol% G+C content. Colonies on Brucella blood agar (BBA) were circular, convex, smooth, grey and small in size. Growth was observed on trypticase soy agar (TSA), TSA +5 % sheep blood and agar. Growth occurred at 18–42 °C on BBA in the presence of 0–3 % NaCl (w/v) and at pH 6.0–8.5. The major polar lipids were phosphatidylethanolamine and phospholipids. The major fatty acids in strain GYB001 were anteiso-C and iso-C 3-OH, and the predominant respiratory quinones were menaquinone-10 (MK-10) and MK-9. The cell wall contained -diaminopimelic acid. Considering these phenotypic features and comparative genome analyses, we propose strain GYB001 as the type strain of sp. nov. (=KCTC 25738=KBN12P06525=LMG 32797).

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  • Accepted:
  • Published Online:
Keyword(s): 16S rRNA , blood , genomics , Parabacteroides and phylogeny
Funding
This study was supported by the:
  • National Research Foundation of Korea (Award NRF-2020R1F1A1066895)
    • Principle Award Recipient: Jung-HyunByun
© 2023 The Authors
  • This is an open-access article distributed under the terms of the Creative Commons Attribution License.
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2023-11-24
2024-05-08
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Parabacteroides leei sp. nov., isolated from human blood
Int J Syst Evol Microbiol 73, 006187 (2023); https://doi.org/10.1099/ijsem.0.006187
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