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INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY logo

Volume 73, Issue 11

sp. nov., an endophytic bacterium isolated from L. in wetland No Access

Abstract

A Gram-stain-positive, aerobic, rod-shaped, non-motile, yellowish and glossy strain, C31, was isolated from a wetland plant L. located south of Poyang Lake, Jiangxi Province, PR China. Phylogenetic analysis based on 16S rRNA gene sequences indicated that strain C31 showed similarity values of lower than 97.0 % to other type species belonging to the genus . The genomic average nucleotide identity values between strain C31 and its reference type species ranged from 68.9–70.9 % and the digital DNA–DNA hybridization values were lower than 27.8 %. The genomic DNA G+C content of strain C31 was 41.9 mol%. The optimal growth temperature, pH and NaCl concentration were 37 °C, pH 7 and 0.5 %, respectively. The major cellular fatty acids (>5.0 %) of strain C31 were anteiso-C (73.7 %), anteiso-C (8.4 %) and iso-C (5.2 %). The polar lipids of strain C31 were diphosphatidylglycerol, phosphatidylglycerol, phosphatidylethanolamine and unidentified phospholipids. The respiratory quinone was MK-7. Based on these phylogenetic and phenotypic characterizations, strain C31 represents a novel species within the genus . Therefore, the proposed name for this newly identified species is sp. nov. and the type strain is C31 (=CCTCC AB 2022349=KCTC 43565).

  • Received:
  • Accepted:
  • Published Online:
Keyword(s): 16S rRNA gene , ANI , Paenibacillus , Polygonum lapathifolium and polyphasic taxonomy
Funding
This study was supported by the:
  • National Key R&D Program of China (Award 2021YFD1500803)
    • Principle Award Recipient: YanshengLi
  • Jilin Province-CAS Special Program for High-Tech Industrialization in Science and Technology Cooperation (Award 2023SYHZ0046)
    • Principle Award Recipient: ZhenhuaYu
  • Strategic Priority Research Program of the Chinese Academy of Sciences (Award XDA28100200)
    • Principle Award Recipient: YanshengLi
© 2023 The Authors
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2023-11-28
2024-05-09
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Paenibacillus polygoni sp. nov., an endophytic bacterium isolated from Polygonum lapathifolium L. in wetland
Int J Syst Evol Microbiol 73, 006185 (2023); https://doi.org/10.1099/ijsem.0.006185
/content/journal/ijsem/10.1099/ijsem.0.006185
/content/journal/ijsem/10.1099/ijsem.0.006185
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