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Volume 73, Issue 12

sp. nov., a novel species isolated from an infected prosthetic aortic graft in a human Open Access

Abstract

A novel bacterial strain, GSTT-20 was isolated from an infected, prosthetic endovascular graft explanted from a shepherd in London, United Kingdom. This strain was an aerobic, catalase-positive, oxidase-negative, Gram-stain-negative, motile, curved rod. It grew on blood agar, chocolate agar and MacConkey agar incubated at 37 °C in an aerobic environment after 48 h, appearing as yellow, mucoid colonies. Analysis of the complete 16S rRNA gene sequence showed closest similarity to with 99.6 % identity and with 99.5 % identity. Phylogenetic analysis of the 16S rRNA gene sequence and phylogenomic analysis of single nucleotide polymorphisms within 1530 core genes showed GSTT-20 forms a distinct lineage in the genus of the family . DNA–DNA hybridization assays against GSTT-20 were estimated at 32.1 % for and 31.9 % for . Genome similarity based on average nucleotide identity was 87.50 % when comparing GSTT-20 to . Based on these results, the strain represented a novel species for which the name sp. nov. was proposed. The type strain is GSTT-20 (NCTC 14621=CECT 30390).

  • Received:
  • Accepted:
  • Published Online:
Keyword(s): Betaproteobacteria , Comamonadaceae , soil bacteria , taxonomy and Variovorax
Funding
This study was supported by the:
  • National Institute for Health and Care Research (Award RJ112/N027)
    • Principle Award Recipient: JonathanD Edgeworth
  • Medical Research Council (Award MR/W025140/1)
    • Principle Award Recipient: LukeBlagdon Snell
  • Medical Research Council (Award MC_PC_19041)
    • Principle Award Recipient: JonathanD Edgeworth
© 2023 The Authors
  • This is an open-access article distributed under the terms of the Creative Commons Attribution License. This article was made open access via a Publish and Read agreement between the Microbiology Society and the corresponding author’s institution.
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2023-12-05
2024-05-09
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Variovorax durovernensis sp. nov., a novel species isolated from an infected prosthetic aortic graft in a human
Int J Syst Evol Microbiol 73, 006184 (2023); https://doi.org/10.1099/ijsem.0.006184
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