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Volume 73, Issue 11

sp. nov., a methylphosphonate-decomposing bacterium isolated from surface seawater in the Xisha Islands, PR China No Access

Abstract

A Gram-stain-negative, facultative anaerobic, methylphosphonate-decomposing, motile by a polar flagellum and rod-shaped marine bacterium, designated S4B1, was isolated from the surface seawater collected from the Yongle Atoll (Xisha Islands, PR China). The pairwise alignment showed the highest sequence similarity of 97.5 and 96.6 % to subsp. 12_122_3T3 and HHS02, respectively. Phylogenetic analysis based on 16S rRNA gene and the phylogenomic analysis of single-copy genes showed that strain S4B1 belonged to the genus and formed a close branch with ZSDZ65. Growth of strain S4B1 occurred at 4–30 °C (optimum, 28 °C), at pH 6.0–8.0 (optimum, pH 7.0) and in the presence of 2–7 % (w/v) NaCl (optimum, 3 %). The predominant fatty acids (>10 %) were C16 : 0, iso-C and summed feature 3 (C 7 or/and C 6). The DNA G+C content of the assembled genomic sequence was 44.3 mol%. Average nucleotide identity (ANI) values between S4B1 and its reference species were lower than the threshold for species delineation (95–96 %), in which its highest ANI value with ZSDZ65 was 87.0 %. DNA–DNA hybridization further showed that strain S4B1 had less than 70 % similarity to its relatives. On the basis of the polyphasic evidence, strain S4B1 is proposed to represent a novel species of the genus , for which the name sp. nov. is proposed. The type strain is S4B1 (=KCTC 92311=MCCC 1K06168).

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  • Accepted:
  • Published Online:
Keyword(s): methylphosphonate , surface seawater , Vibrio methylphosphonaticus sp. nov. and Xisha Islands
Funding
This study was supported by the:
  • Fund of Key Laboratory of Marine Ecological Conservation and Restoration, Ministry of Natural Resources/ Fujian Provincial Key Laboratory of Marine Ecological Conservation and Restoration (Award EPR2023001)
    • Principle Award Recipient: XiaoleiWang
  • National Key Research and Development Program of China (Award 2021YFC3100503)
    • Principle Award Recipient: WentaoNiu
  • National Natural Science Foundation of China (Award 42006085)
    • Principle Award Recipient: XiaoleiWang
  • Marine S&T Fund of Fujian Province (Award 2021J05094)
    • Principle Award Recipient: XiaoleiWang
© 2023 The Authors
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/content/journal/ijsem/10.1099/ijsem.0.006183
2023-11-24
2024-05-08
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Vibrio methylphosphonaticus sp. nov., a methylphosphonate-decomposing bacterium isolated from surface seawater in the Xisha Islands, PR China
Int J Syst Evol Microbiol 73, 006183 (2023); https://doi.org/10.1099/ijsem.0.006183
/content/journal/ijsem/10.1099/ijsem.0.006183
/content/journal/ijsem/10.1099/ijsem.0.006183
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