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INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY logo International Journal of Systematic and Evolutionary Microbiology

Volume 73, Issue 11

gen. nov., sp. nov., a novel bacterium isolated from activated sludge, and proposal of fam. nov. No Access

Abstract

A novel Gram-stain-negative, long rod-shaped, aerobic, non-motile, non-spore-forming and orange-pigmented bacterium, designated LB-30, was isolated from activated sludge. Growth was observed at 15–40 °C (optimum 30 °C), pH 6.0–9.0 (optimum 7.0) and salinities of 0–3.0 % (w/v; optimum 2.0 %). LB-30 showed less than 89.9 % sequence similarities to the recognized taxa of the order . The results of phylogenetic analysis based on 16S rRNA gene sequences and phylogenomic tree indicated that LB-30 formed a distinct lineage within the order . The average amino acid identity (AAI) values between LB-30 and members of the related families Cyclobacteriaceae, Fulvivirgaceae, Roseivirgaceae, Reichenbachiellaceae, Cesiribacteraceae, and Hymenobacteraceae in the order were 50.5–54.6 %. The sole respiratory quinone of LB-31 was menaquinone 7 (MK-7). The major polar lipids were phosphatidylethanolamine, aminolipid and four unidentified lipids. The major fatty acids were iso-C, iso-CG and iso-C 3-OH. The DNA G+C content was 43.8 mol%, calculated from the genome sequence. On the basis of differences in the phenotypic, physiological and biochemical characteristics, and distinct phylogenetic relationships, strain LB-30 is proposed to represent a novel species in a novel genus for which the name gen. nov., sp. nov is proposed, within a novel family fam. nov. of the order . The type strain is LB-30 (=GDMCC 1.3629= KCTC 92689).

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  • Accepted:
  • Published Online:
Keyword(s): 16S rRNA gene , Bacteroidota , Shiella aurantiaca and taxonomy
Funding
This study was supported by the:
  • Special fund for science and technology program of Jiangsu province (Award BM2022019)
    • Principal Award Recipient: QirongShen
  • Natural Science Foundation of Jiangxi Province (Award 20224BAB215005)
    • Principal Award Recipient: BinLiu
© 2023 The Authors
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2023-11-22
2025-12-11

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Shiella aurantiaca gen. nov., sp. nov., a novel bacterium isolated from activated sludge, and proposal of Shiellaceae fam. nov.
Int J Syst Evol Microbiol 73, 006175 (2023); https://doi.org/10.1099/ijsem.0.006175
/content/journal/ijsem/10.1099/ijsem.0.006175
/content/journal/ijsem/10.1099/ijsem.0.006175
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