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INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY logo

Volume 73, Issue 11

f.a., sp. nov. and f.a., sp. nov., two novel ascomycetous yeast species from mushrooms and cocoa leaves in Thailand, and reassignment of , and to the genus No Access

Abstract

Six strains representing two novel ascomycetous yeast species were isolated from mushroom fruiting bodies and cocoa leaves collected in Thailand. Analysis of the internal transcribed spacer (ITS) regions and the D1/D2 domains of the large subunit rRNA gene sequences showed that the six strains were divided into two groups. The first group consisted of four strains (DMKU-SSK46, DMKU-SK1, SCCL3-5 and SCCL19-3), that were closely related to the type strains of , , , and , but with 12–14 (2.5–2.9 %) and 28–50 (5.4–8.8 %) nucleotide substitutions in the D1/D2 domains and the ITS regions, respectively. However, two strains (DMKU-KMY40 and DMKU-KO18) of the second group differed from a group of described species, , , , , , and by 8–15 (1.5–2.8 %) and 45–53 (8.2–9.6 %) nucleotide substitutions in the D1/D2 domains and the ITS regions, respectively. Phylogenetic analysis based on the concatenated sequences of the ITS regions and D1/D2 domains showed that these strains represented two species of the clade that were distinct from the other related species. Based on the phylogenetic analysis and phenotypic characteristics, these six strains were assigned to two novel species of the genus , although formation of ascospores was not observed. f.a., sp. nov., is proposed for the first group (four strains). The holotype is TBRC 17139 (ex-type culture: PYCC 9797). The MycoBank number is MB 849637. f.a., sp. nov. is proposed for the second group (two strains). The holotype is TBRC 14868 (ex-type culture: PYCC 8907). The MycoBank number is MB 849638. In addition, it is proposed that , and are reassigned to the genus as comb. nov., comb. nov. and comb. nov., respectively.

  • Received:
  • Accepted:
  • Published Online:
Keyword(s): leaves , mushroom , Thailand , Yamadazyma koratensis , Yamadazyma sisaketensis and yeast
Funding
This study was supported by the:
  • Kasetsart University Research and Development Institute (Award YF(KU)19.65)
    • Principle Award Recipient: PannidaKhunnamwong
© 2023 The Authors
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/content/journal/ijsem/10.1099/ijsem.0.006174
2023-11-22
2024-05-09
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Yamadazyma sisaketensis f.a., sp. nov. and Yamadazyma koratensis f.a., sp. nov., two novel ascomycetous yeast species from mushrooms and cocoa leaves in Thailand, and reassignment of Candida andamanensis, Candida jaroonii and Candida songkhlaensis to the genus Yamadazyma
Int J Syst Evol Microbiol 73, 006174 (2023); https://doi.org/10.1099/ijsem.0.006174
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