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Volume 73, Issue 11

sp. nov., isolated forest soil No Access

Abstract

A milky-white-coloured, aerobic, Gram-stain-positive, rod-shaped and motile bacterial strain (GW78) was isolated from forest soil. GW78 was catalase-positive and oxidase-negative. The strain was able to grow optimally at 37 °C and at pH 7.0 in Reasoner's 2A media. The phylogenetic and 16S rRNA gene sequence analysis of GW78 showed its affiliation with the genus . The 16S rRNA gene sequence of GW78 revealed 98.3 % similarity to its nearest neighbour VKPM B-7519. Its chemotaxonomic properties included MK-7 as the sole menaquinone, phosphatidylglycerol, diphosphatidylglycerol, phosphatidylmonomethylethanolamine and phosphatidylethanolamine as major polar lipids, and anteiso-C, C 11 and anteiso-C as predominant fatty acids. Digital DNA–DNA hybridization and average nucleotide identity results with its closest relatives were <74.0 % and <14.0 %, respectively. Overall, 16S rRNA gene sequence comparisons, phylogenetic and genomic evidence, and phenotypic and chemotaxonomic data allow the differentiation of GW78 from other members of the genus . Thus, we propose that strain GW78 represents a novel species of the genus , with the name sp. nov. The type strain is GW78 (=KCTC 43430=NBRC 116023).

  • Received:
  • Accepted:
  • Published Online:
Keyword(s): forest soil , new prokaryotic taxa , Paenibacillaceae , Paenibacillus caseinilyticus sp. nov. and phylogeny
Funding
This study was supported by the:
  • National Institute of Biological Resources (Award NIBR202203112)
    • Principle Award Recipient: Dong-UkKim
© 2023 The Authors
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2023-11-20
2024-05-08
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Paenibacillus caseinilyticus sp. nov., isolated forest soil
Int J Syst Evol Microbiol 73, 006171 (2023); https://doi.org/10.1099/ijsem.0.006171
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