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INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY logo

Volume 73, Issue 11

sp. nov. and sp. nov., isolated from No Access

Abstract

Four yellow-coloured strains (zg-Y815/zg-Y108 and zg-Y859/zg-Y826) were isolated from the intestinal contents of and assigned to the ' group'. The four strains grew optimally on brain heart infusion agar with 5 % defibrinated sheep blood plate at 30 °C, pH 7.0 and with 0.5 % NaCl (w/v). Comparative analysis of their 16S rRNA genes indicated that the two strain pairs belong to the genus , showing the highest similarity to 785 (99.52 %), which was further confirmed by the 16S rRNA gene and genome-based phylogenetic analysis. The comparative genomic analysis [digital DNA–DNA hybridization, (dDDH) and average nucleotide identity (ANI)] proved that the four strains are two different species (zg-Y815/zg-Y108, 71.7 %/96.8 %; zg-Y859/zg-Y826, 87.3 %/98.5 %) and differ from other known species within the genus (zg-Y815, 19.6–32.3 %/77.2–88.0 %; zg-Y859, 19.5–29.3 %/77.4–86.3 %). Strain pairs zg-Y815/zg-Y108 and zg-Y859/zg-Y826 had the same major cellular fatty acids (iso-C and anteiso-C), with MK-8(H) as their dominant respiratory quinone (70.6 and 61.7 %, respectively). The leading polar lipids were diphosphatidylglycerol, phosphatidylglycerol, and phosphatidylinositol. The detected amino acids and cell-wall sugars of the two new species were identical (amino acids: alanine, glutamic acid, and lysine; sugars: rhamnose, galactose, mannose, glucose, and ribose). According to the phylogenetic, phenotypic, and chemotaxonomic analyses, we concluded that the four new strains represented two different novel species in the genus , for which the names sp. nov. (zg-Y815= GDMCC 1.3494 = JCM 35821) and sp. nov. (zg-Y859 = GDMCC 1.3493 = JCM 35822) are proposed.

  • Received:
  • Accepted:
  • Published Online:
Keyword(s): ANI , Arthrobacter , dDDH , Novel species and Phylogenetic analysis
© 2023 The Authors
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/content/journal/ijsem/10.1099/ijsem.0.006168
2023-11-29
2024-05-08
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Arthrobacter zhaoxinii sp. nov. and Arthrobacter jinronghuae sp. nov., isolated from Marmota himalayana
Int J Syst Evol Microbiol 73, 006168 (2023); https://doi.org/10.1099/ijsem.0.006168
/content/journal/ijsem/10.1099/ijsem.0.006168
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