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Volume 73, Issue 11

sp. nov., isolated from Iru, fermented African locust beans Open Access

Abstract

A Gram-positive, motile, aerobic, rod-shaped, endospore-forming strain designated IRB4-01 was isolated from fermented African locust beans (Iru) obtained from Bodija market in the city of Ibadan, southwestern Nigeria, during a screening process from food-related sources. IRB4-01 grew at 10–50 °C (optimum, 35–37 °C), pH 6–10 (optimum, pH 7) and in 0–6 % NaCl (optimum, 1–3 %). Phylogenetic analyses based on 16S rRNA and combined short- and long-read genome sequencing revealed that IRB4-01 is closely related to SYSU K30005 and 10a. The cell-wall peptidoglycan type was A4α (Lys–Asp), containing the diagnostic diamino acid lysine. The major polar lipids in strain IRB4-01 were diphosphatidylglycerol, phosphatidylethanolamine, phosphatidylglycerol and an unidentified phospholipid, while the predominant menaquinone was MK-7. The major cellular fatty acids were iso-C, anteiso-C and iso-C. Genomic DNA G+C content was 37.4  mol%, while the digital DNA–DNA hybridization revealed 33.6 and 32.3 % relatedness to SYSU K30005 and 10a, respectively. Based on phenotypic, physiological and chemotaxonomic characteristics, as well as genome comparisons, strain IRB4-01 represents a novel species of the genus , for which the name sp. nov. is proposed. The type strain is IRB4-01 (NCIMB 15452=LMG 32887). Hybrid genome data are provided on the NCBI database using the Bioproject number PRJNA906010 and accession numbers CP113527 and CP113528. Additionally, a representative 16S rRNA sequence is available with the GenBank accession number OQ566940.

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  • Accepted:
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Keyword(s): biotechnology , food , genome analysis and Lysinibacillus
Funding
This study was supported by the:
  • DAAD (Award STIBET Doktoranden)
    • Principle Award Recipient: StephenOlusanmi Akintayo
  • Bayer Foundation (Award F-2018-BS-0260)
    • Principle Award Recipient: StephenOlusanmi Akintayo
© 2023 The Authors
  • This is an open-access article distributed under the terms of the Creative Commons Attribution License.
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2023-11-09
2024-05-08
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Lysinibacillus irui sp. nov., isolated from Iru, fermented African locust beans
Int J Syst Evol Microbiol 73, 006167 (2023); https://doi.org/10.1099/ijsem.0.006167
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