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INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY logo

Volume 73, Issue 11

gen. nov., sp. nov., a novel member of the family isolated from a hydrothermal zone No Access

Abstract

A Gram-stain-negative, aerobic, non-motile and rod-shaped bacterial strain, designated as strain TK19130, was isolated from the Lonqi hydrothermal zone in the Southwest Indian Ridge. Growth occurred with 1–12 % (w/v) NaCl (optimum, 2–4 %), at 10–40 °C (optimum, 30–35 °C) and at pH 6.0–9.0 (optimum, pH 7.0–8.0). The genome of strain TK19130 was 3.15 Mb, with a DNA G+C content of 41.35 %. Based on the results of 16S rRNA gene sequence analysis, strain TK19130 was affiliated with the family , in which the highest similarity was 90.54 % to A6D-50, under the genus demarcation boundary (94.50 %). Average nucleotide identity values between strain TK19130 and adjacent strains were 67.17–72.00 %, lower than the recommended threshold of 73.98 % for genus delineation. The predominant respiratory quinone of strain TK19130 was menaquinone 6. Major polar lipids were phosphatidylethanolamine, three aminolipids and one unidentified polar lipid. Major fatty acids were detected as iso-C G, iso-C and iso-C 3-OH. Based on the polyphasic taxonomic evidence presented above, strain TK19130 formed an independent branch representing a new species of a novel genus within the family , for which the name gen. nov., sp. nov. is proposed. The type strain is TK19130 (=CGMCC 1.18993=JCM 35842=MCCC M28200).

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  • Accepted:
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Keyword(s): hydrothermal zone , polyphasic taxonomic approach and Thermobacterium salinum
Funding
This study was supported by the:
  • XMU Training Program of Innovation and Entrepreneurship for Undergraduates (Award 2022X0968)
    • Principle Award Recipient: BeihanChen
  • XMU Undergraduate Innovation and Entrepreneurship Training Programs (Award KFJJ-202218)
    • Principle Award Recipient: BeihanChen
  • International Science Partnership Programme of the Chinese Academy of Sciences (Award 121311KYSB20190029)
    • Principle Award Recipient: KaiTang
  • National Natural Science Foundation of China (Award 42276120)
    • Principle Award Recipient: KaiTang
  • National Natural Science Foundation of China (Award 42076160)
    • Principle Award Recipient: KaiTang
© 2023 The Authors
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2023-11-15
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Thermobacterium salinum gen. nov., sp. nov., a novel member of the family Flavobacteriaceae isolated from a hydrothermal zone
Int J Syst Evol Microbiol 73, 006166 (2023); https://doi.org/10.1099/ijsem.0.006166
/content/journal/ijsem/10.1099/ijsem.0.006166
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