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INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY logo

Volume 73, Issue 11

gen. nov., sp. nov., isolated from marine sediment, and genomic comparison with multiple genera No Access

Abstract

A Gram-stain-negative, rod-shaped, non-gliding, non-flagellated, yellow, facultatively aerobic bacterial strain, designated as W260, was isolated from marine sediment of Xiaoshi Island, Weihai, PR China. The cells of W260 were 0.3–0.5 µm wide and 1.5–2.0 µm long. Strain W260 grows optimally at a temperature of 33 °C (range, 15–37 °C), pH 8 (range, pH 6.5–9.5) and witha NaCl concentration of 3.0 % (w/v; range, 1–8 %). It has the highest sequence similarity to DSM 21152 (91.7 %), followed by MCCC 1K00261 (91.4 %) and DSM 17116 (90.7 %). The similarity between strain W260 and the species DSM 16326 was 89.4 %. Genome sequencing revealed a genome size of 3 430 000 bp and a DNA G+C content of 64.5 mol%. The average nucleotide identity and digital DNA–DNA hybridization values between strain W260 and MCCC 1K00261 were 69.6 and 16.1–20.6 %, respectively. The predominant quinone was ubiquitin-8, and the major fatty acids were iso-C and iso-C. The polar lipids consisted of phosphatidylethanolamine, phospholipid, phosphatidylglycerol, diphosphatidylglycerol and four unidentified lipids. Based on phenotypic, phylogenetic and chemotaxonomic information, it was determined that strain W260 represents a novel genus and species and it was given the name sp. nov. The type strain is W260 (=MCCC 1H00471=KCTC 92639).

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Keyword(s): 16S rRNA gene , genome , Marinihelvus fidelis and phylogenetic analysis
Funding
This study was supported by the:
  • National Natural Science Foundation of China (Award 32070002)
    • Principle Award Recipient: Zong-JunDu
  • Science & Technology Fundamental Resources Investigation Program (Award 2019FY100700)
    • Principle Award Recipient: Zong-JunDu
© 2023 The Authors
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2023-11-20
2024-05-09
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Marinihelvus fidelis gen. nov., sp. nov., isolated from marine sediment, and genomic comparison with multiple genera
Int J Syst Evol Microbiol 73, 006165 (2023); https://doi.org/10.1099/ijsem.0.006165
/content/journal/ijsem/10.1099/ijsem.0.006165
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