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INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY logo International Journal of Systematic and Evolutionary Microbiology

Volume 73, Issue 11

gen. nov., sp. nov., a hydrogenotrophic methanogen, isolated from a submarine mud volcano in the offshore area of southwestern Taiwan No Access

Abstract

A novel mesophilic, hydrogenotrophic methanogen, strain CYW5, was isolated from a sediment sample of a piston core collected from submarine mud volcano MV5 located in the offshore area of southwestern Taiwan. Cells of strain CYW5 were irregular coccids, 0.5–1.0 µm in diameter and lysed easily by 0.01 % sodium dodecyl sulphate (SDS) treatment. Strain CYW5utilized formate or hydrogen plus carbon dioxide as catabolic substrates for methanogenesis. The optimal growth conditions were 37 °C, 0.043–0.085 M NaCl and pH 6.02–7.32. The genomic DNA G+C content calculated from the genome sequence of strain CYW5 was 56.2 mol%. The results of phylogenetic analysis of 16S rRNA gene sequences indicated that strain CYW5 represented a member of the family in the order , and was closely related to the members of the genus . The most closely related species was JR1 (94.9 % of 16S rRNA gene sequence identity). The average nucleotide identity and average amino acid identity values between strain CYW5 and members of the family were 74.7–78.5 % and 49.1–64.9%, respectively. Although many of the morphological and physiological characteristics of strain CYW5 and the species of the genus were similar, they were distinguishable by the differences in genomic G+C content and temperature, NaCl and pH ranges for growth. Based on these phenotypic, phylogenetic and genomic results, we propose that strain CYW5 represents a novel species, of a novel genus, named gen. nov., sp. nov. The type strain is CYW5 (=BCRC AR10048=DSM 100756=NBRC 111404).

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Keyword(s): archaea , methanogen , Methanovulcanius and submarine mud volcano
Funding
This study was supported by the:
  • Sanming University (Award 20YG09)
    • Principal Award Recipient: Sheng-ChungChen
  • Ministry of Science and Technology, Taiwan, R.O.C. (Award MOST 103~107-3113-M-005-001)
    • Principal Award Recipient: Mei-ChinLai
  • Central Geological Survey, Ministry of Economic Affairs (Award 99-5226904000-04-03)
    • Principal Award Recipient: Mei-ChinLai
© 2023 The Authors
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2023-11-08
2026-02-07

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Methanovulcanius yangii gen. nov., sp. nov., a hydrogenotrophic methanogen, isolated from a submarine mud volcano in the offshore area of southwestern Taiwan
Int J Syst Evol Microbiol 73, 006164 (2023); https://doi.org/10.1099/ijsem.0.006164
/content/journal/ijsem/10.1099/ijsem.0.006164
/content/journal/ijsem/10.1099/ijsem.0.006164
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