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Volume 73, Issue 11

sp. nov., isolated from Scandinavian patients with seal finger or septic arthritis after contact with seals Open Access

Abstract

Seal finger (sealer’s finger, spekk finger), an extremely painful hand infection contracted by individuals handling seals, has previously been associated with . From 2000 to 2014, six independent strains of a novel species were isolated at Statens Serum Institut, Denmark, from Scandinavian patients with seal finger (M5725, M6447, M6620, M6642 and M6879) or septic arthritis (M6921). Prior to the onset of infection, all patients had reported contact with unspeciated seals. All isolates grew within 2–5 days in Friis’ modified broth and metabolized glucose and arginine but not urea. Strains M5725, M6447, M6642 and M6921 also grew in Hayflick-type media. Colonies on agar media were large (0.5–1.0 mm) and had a typical ‘fried egg’ appearance, reduced tetrazolium, and were digitonin sensitive. Growth occurred at 32 °C but not at 42 °C. Strains were susceptible to doxycycline and moxifloxacin but resistant to azithromycin and erythromycin. The genomes of the six strains were sequenced and relatedness to all known species was inferred. Phylogenetic analyses using 16S rRNA gene sequences and core genome single nucleotide polymorphisms showed that the isolated strains were highly similar and phylogenetically distinct from all other species within the genus . The sizes of the genome sequences of the strains ranged from 744 321 to 772409 bp, with a G+C content of 25.0–25.2 mol%. Based on these analyses, we propose a novel species of the genus with the name sp. nov. with the first isolate M5725 (NCTC 14922=DSM 116188) as the proposed type strain and representative strains M6447, M6620, M6642, M6879 and M6921.

  • Received:
  • Accepted:
  • Published Online:
Keyword(s): Mycoplasma , panaritium , PCR , phocimorsus , seal finger and whole-genome sequencing
© 2023 The Authors
  • This is an open-access article distributed under the terms of the Creative Commons Attribution License.
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2023-11-15
2024-05-08
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Mycoplasma phocimorsus sp. nov., isolated from Scandinavian patients with seal finger or septic arthritis after contact with seals
Int J Syst Evol Microbiol 73, 006163 (2023); https://doi.org/10.1099/ijsem.0.006163
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