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INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY logo

Volume 73, Issue 11

sp. nov., isolated from pickled potherb mustard ( Coss.) Open Access

Abstract

Strain BD7642 was isolated from Chinese pickled potherb mustard ( Coss.) purchased from a local market in Shanghai, PR China. A polyphasic approach, including 16S rRNA gene sequence, housekeeping gene, average nucleotide identity (ANI), digital DNA–DNA hybridization (dDDH), G+C content and phenotypic analyses, was employed to characterize strain BD7642. Cells of the bacterium were short round rods, Gram-stain-positive, non-spore-forming and catalase-negative. The strain grew at 30–45 °C and pH 4.0–8.0. Optimum growth occurred at 35–40 °C and pH 6.0–7.0. The strain exhibited growth with salt (NaCl) concentrations of up to 5 % (w/v). The G+C content of the strain’s genomic DNA was 31.37 mol%. The major fatty acids were C, Cc9 and summed feature 10 (Cc11/t9/t6). 16S rRNA gene sequencing revealed that strain BD7642 represents a member of the genus and it had high sequence similarity to NBRC 102162 (96.73 %), LGM 23560 (96.66 %) and JCM 1231 (95.82 %). The dDDH values between strain BD7642 and its phylogenetically related species within the genus ranged from 12.6 to 25.4 %. The ANI values between strain BD7642 and its closely related taxa were far lower than the threshold (95 %–96 %) used for species differentiation. Results of phylogenetic, physiological and phenotypic characterization confirmed that strain BD7642 represents a novel species within the genus , for which the name sp. nov. is proposed. The type strain is BD7642 (=CCTCC AB 2022398=JCM 36074).

  • Received:
  • Accepted:
  • Published Online:
Keyword(s): bile salt hydrolase , Ligilactobacillus , novel species and pickled potherb mustard
Funding
This study was supported by the:
  • Shanghai Municipal State-owned Assets Supervision and Administration Commission (Award No. 2022013)
    • Principle Award Recipient: liuzhenmin
  • Key Technologies Research and Development Program (Award 2022YFD2100704)
    • Principle Award Recipient: zhengjunwu
© 2023 The Authors
  • This is an open-access article distributed under the terms of the Creative Commons Attribution License.
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2023-11-10
2024-05-08
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Ligilactobacillus cholophilus sp. nov., isolated from pickled potherb mustard (Brassica juncea Coss.)
Int J Syst Evol Microbiol 73, 006160 (2023); https://doi.org/10.1099/ijsem.0.006160
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