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INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY logo

Volume 73, Issue 11

Two novel alkalitolerant species sp. nov. and sp. nov. No Access

Abstract

In this study, two novel alkalitolerant strains (FJAT-53046 and FJAT-53715) were isolated from sediment samples collected in Zhangzhou, PR China. Phylogeny based on 16S rRNA gene sequences suggested that strains FJAT-53046 and FJAT-53715 were new members of the genus . The two novel strains showed the highest 16S rRNA gene sequence similarity to DSM 16206, with values of 97.4 and 97.6 %, respectively. The average nucleotide identity (ANI) and digital DNA–DNA hybridization (dDDH) values between the two strains and the reference strain were 77.2 and 79.6 %, 20.9 and 30.2 %, respectively, which were below the prokaryotic species delineation thresholds. The ANI and dDDH values between strains FJAT-53046 and FJAT-53715 were 86.0 and 30.2 %, respectively, suggesting that they belonged to different species in the genus . The major respiratory quinone in both strains was MK-7 and the major cellular fatty acids were anteiso-C and anteiso-C. Diphosphatidylglycerol, phosphatidylglycerol and phosphatidylethanolamine were the major polar lipids in both novel strains. Combined with results stemming from the determination of physical and biochemical characteristics, chemical properties, and genome analysis, strains FJAT-53046 and FJAT-53715 are proposed to represent two novel species of the genus , for which the names sp. nov. and sp. nov. are proposed. The type strains are FJAT-53046 (=GDMCC 1.3077=JCM 35611) and FJAT-53715 (=GDMCC 1.3076=JCM 35610), respectively.

  • Received:
  • Accepted:
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Keyword(s): alkalitolerant , mangrove ecosystem , Pseudalkalibacillus sedimenti sp. nov. and Pseudalkalibacillus spartinae sp. nov.
Funding
This study was supported by the:
  • Fujian Academy of Agricultural Sciences (Award GJYS202203)
    • Principle Award Recipient: Guo-HongLiu
  • National Natural Science Foundation of China (Award 42007221)
    • Principle Award Recipient: Guo-HongLiu
© 2023 The Authors
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2023-11-02
2024-05-08
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Two novel alkalitolerant species Pseudalkalibacillus spartinae sp. nov. and Pseudalkalibacillus sedimenti sp. nov.
Int J Syst Evol Microbiol 73, 006159 (2023); https://doi.org/10.1099/ijsem.0.006159
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