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INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY logo

Volume 73, Issue 11

Characterization of sp. nov. and reclassification of a species based on a taxogenomic approach No Access

Abstract

An extremely halophilic archaeon, strain S1AR25-5A, was isolated from a hypersaline soil sampled in Odiel Saltmarshes Natural Area (Huelva, Spain). The cells were Gram-stain-negative, motile, pleomorphic rods. Cell growth was observed in the presence of 15–30 % (w/v) NaCl [optimum, 25 % (w/v) NaCl], at pH 6.0–9.0 (optimum, pH 6.5–7.5) and at 25–50 °C (optimum, 37 °C). Based on the 16S rRNA and ′ gene sequence comparisons, strain S1AR25-5A was affiliated to the genus . Taxogenomic analysis, including comparison of the genomes and the phylogenomic tree based on the core-orthologous proteins, together with the genomic indices, i.e., orthologous average nucleotide identity, digital DNA–DNA hybridization and average amino acid identity, confirmed that strain S1AR25-5A (=CCM 9249=CECT 30619) represents a new species of the genus , for which we propose the name sp. nov. The major polar lipids were phosphatidylglycerol, phosphatidylglycerol phosphate methyl ester, phosphatidylglycerol sulphate and an unidentified glycolipid, which correlated with the lipid profile of species of the genus . In addition, based on the modern approach in description of species in taxonomy of prokaryotes, the above mentioned genomic indexes indicated that the species should be considered as a heterotypic synonym of .

  • Received:
  • Accepted:
  • Published Online:
Keyword(s): haloarchaea , Haloarcula , heterotypic synonym , hypersaline soils , taxogenomic analysis and taxonomy
Funding
This study was supported by the:
  • Consejería de Economía, Conocimiento, Empresas y Universidad, Junta de Andalucía (Award P20_01066 and BIO-213)
    • Principle Award Recipient: AntonioVentosa
  • Ministerio de Ciencia, Innovación y Universidades (Award PID2020-118136GB-I00 funded by MCIN/AEI/10.13039/501100011033)
    • Principle Award Recipient: AntonioVentosa
© 2023 The Authors
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Characterization of Haloarcula terrestris sp. nov. and reclassification of a Haloarcula species based on a taxogenomic approach
Int J Syst Evol Microbiol 73, 006157 (2023); https://doi.org/10.1099/ijsem.0.006157
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