1887

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Volume 73, Issue 11

sp. nov. and sp. nov., isolated from coastal sediment No Access

Abstract

Two novel rod-shaped, Gram-stain-negative, aerobic and non-motile bacterial strains, designated M39 and C2-7, were isolated from the coastal sediment of Xiaoshi Island, Weihai, PR China. Growth of strain M39 occurred at 15–37 °C, at pH 6.0–9.0 and in the presence of 1.0–9.0 % (w/v) NaCl. Strain C2-7 grew at 15–40 °C, at pH 6.0–8.0 and in the presence of 0.5–8.0 % (w/v) NaCl. Phylogenetic analysis based 16S rRNA gene sequences revealed that strains M39 and C2-7 belong to the phylum . Based on the results of 16S rRNA gene sequence analysis, the closest relative of strain M39 was KCTC 92035 (95.4 %), and the closest relative of strain C2-7 was DPG-3 (97.0 %). The percentage of conserved protein and average nucleotide identity values between strain M39 and some species of the genus were 66.9–77.6% and 69.3–71.0 %, respectively, while those between strain C2-7 and some species of the genus were 68.0–70.1% and 56.1–72.6 %, respectively. The major cellular fatty acids (>10 %) of strain M39 consisted of iso-C F, iso-C and iso-C 3-OH, while those of strain C2-7 were iso-C and C ω7/C ω6. MK-6 was the only respiratory quinone that was compatible with the genus of strain M39. The predominant menaquinone of strain C2-7 was MK-7. The major polar lipids of strain M39 were phosphatidylethanolamine and glycolipids, and those of strain C2-7 were phosphatidylethanolamine, one unidentified aminolipid and four unidentified lipids. The DNA G+C contents of strains M39 and C2-7 were 46.9 and 40.8 mol%, respectively. Based upon the results presented in this study, strains M39 and C2-7 represent novel species of the genera and , respectively, for which the names sp. nov. and sp. nov. are proposed with the type strains M39 (=MCCC 1H00498=KCTC 92014) and C2-7 (=MCCC 1H00414=KCTC 92027).

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Keyword(s): 16S rRNA gene , Algoriphagus , polyphasic taxonomy and Robiginitalea
Funding
This study was supported by the:
  • National Natural Science Foundation of China (Award 32070002)
    • Principle Award Recipient: Zong-JunDu
  • Science & Technology Fundamental Resources Investigation Program (Award 2019FY100700)
    • Principle Award Recipient: Zong-JunDu
© 2023 The Authors
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2023-11-09
2024-05-08
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Robiginitalea aurantiaca sp. nov. and Algoriphagus sediminis sp. nov., isolated from coastal sediment
Int J Syst Evol Microbiol 73, 006155 (2023); https://doi.org/10.1099/ijsem.0.006155
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