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INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY logo

Volume 73, Issue 11

sp. nov. and sp. nov., isolated from streams in China and reclassification of as a synonym of No Access

Abstract

Three Gram-stain-negative, aerobic, rod-shaped, non-motile strains (LYT7W, DC10W and LFS242W) were isolated from streams in PR China. Comparisons based on the 16S rRNA gene sequences showed that these three strains showed high 16S rRNA gene sequence similarity to GFA-11 (99.2, 98.8 and 99.8 %, respectively) and JC289 (98.8, 98.6 and 99.4 %, respectively), and less than 96.6 % to other species of the genus . The phylogenetic tree reconstructed based on the 16S rRNA gene sequences showed that strains LYT7W, DC10W, LFS242W, GFA-11 and JC289 formed a tight cluster. The phylogenomic tree also supported the above robust phylogenetic relationships. The calculated OrthoANIu and digital DNA–DNA hybridization values between strains LYT7W and DC10W were 95.2 % and 61.9 %, respectively. Although these values are located in the transition region for species demarcation, the similar physiological and genotypic characteristics supported that strains LYT7W and DC10W should belong to the same species. The pairwise OrthoANIu and digital DNA–DNA hybridization values between strain LFS242W and its related strains were less than 91.8 and 45.3 %, respectively, indicating that strain LFS242W should represent an independent novel species of the genus . It should be noticed that the pairwise OrthoANIu and digital DNA–DNA hybridization values between strains LMG 24501 and KCTC 42575 were 96.9 and 73.1 %, respectively. The similar physiological and genotypic characteristics also supported that Ramaprasad . 2015 should be a synonym of Sheu . 2009. Combining the above descriptions, strains LYT7W, DC10W and LFS242W should represent two novel species of the genus , for which the names sp. nov. (type strain LYT7W=GDMCC 1.3222=KCTC 92561) and sp. nov. (type strain LFS242W=GDMCC 1.3223=KCTC 92562) are proposed.

  • Received:
  • Accepted:
  • Published Online:
Keyword(s): Flectobacillus and streams
Funding
This study was supported by the:
  • Yunnan Provincial Ministry of Science and Technology (Award 202301AT070100)
    • Principle Award Recipient: HuibinLu
  • Yunnan Provincial Ministry of Science and Technology (Award 202203AC100002-02, 202005AF150005, 202305AM070002)
    • Principle Award Recipient: GuangjieChen
© 2023 The Authors
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2023-11-02
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Flectobacillus longus sp. nov. and Flectobacillus rivi sp. nov., isolated from streams in China and reclassification of Flectobacillus rhizosphaerae as a synonym of Flectobacillus roseus
Int J Syst Evol Microbiol 73, 006148 (2023); https://doi.org/10.1099/ijsem.0.006148
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