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Abstract

Two halophilic archaeal strains, ZS-10 and GSL13, were isolated from the Zhoushan marine saltern in Zhejiang, and an inland saline soil from the Tarim Basin, Xinjiang, PR China, respectively. The cells of strain ZS-10 were pleomorphic while those of strain GSL13 were rod-shaped. Both of them stained Gram-negative and formed red-pigmented colonies on agar plates and their cells lysed in distilled water. The optimum growth of strain ZS-10 was observed at 40 °C, 3.4 M NaCl, 0.03 M MgCl and pH 7.5, while that of strain GSL13 was at 37 °C, 3.1 M NaCl, 0.5 M MgCl and pH 7.5. Phylogenetic and phylogenomic analyses indicated that these two strains were related to and , respectively. Strains ZS-10 and GSL13 could be differentiated from the current members of and based on the comparison of diverse phenotypic characteristics. The average amino acid identity, average nucleotide identity and digital DNA–DNA hybridization values among strain ZS-10 and current species of were 75.8–78.6 %, 80.6–81.9 % and 24.3–26.1 %, respectively. These values between strain GSL13 and current species of were 78.4–80.8 %, 79.8–82.8% and 22.7–25.7 %, respectively, clearly below the threshold values for species demarcation. The polar lipids of strain ZS-10 were phosphatidylglycerol (PG), phosphatidylglycerol phosphate methyl ester (PGP-Me) and sulphated mannosyl glucosyl diether (S-DGD-1), while those of strain GSL13 were phosphatidic acid, PG, PGP-Me, phosphatidylglycerol sulphate and S-DGD-1. The polar lipid profile of strain GSL13 was identical to those of , whereas strain ZS-10 did not contain the minor glycolipids detected in the current species. The phenotypic, phylogenetic and genome-based results suggested that strains ZS-10 (=CGMCC 1.12868=JCM 30241) and GSL13 (=CGMCC 1.15214=JCM 30841) represent two novel species, for which the names sp. nov. and sp. nov. are proposed.

Funding
This study was supported by the:
  • National Natural Science Foundation of China (Award 32070003)
    • Principle Award Recipient: Heng-LinCui
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2023-11-02
2024-07-20
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