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INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY logo

Volume 73, Issue 11

sp. nov., an obligately chemolithoautotrophic, hydrogen-oxidizing bacterium isolated from coastal marine sediments No Access

Abstract

A novel mesophilic, chemolithoautotrophic, hydrogen-oxidizing bacterium, designated strain ST1-3, was isolated from mud sediment samples collected from mangroves in Jiulong River estuary. The cells were Gram-stain-negative, non-motile and rod-shaped. The temperature, pH and salinity ranges for growth of strain ST1-3 were 4–45 °C (optimum, 35 °C), pH 5.0–8.5 (optimum, pH 7.0) and 0–8.0 % (w/v) NaCl (optimum, 4.0 %). The isolate was an obligate chemolithoautotroph capable of growth using hydrogen as the only energy source, and molecular oxygen, thiosulphate and elemental sulphur as electron acceptors. The major cellular fatty acids of strain ST1-3 were summed feature 3 (C 7 and/or C 6), C and summed feature 8 (C 7). The major polar lipids were phosphatidylethanolamine, phosphatidyldimethyl ethanolamine and phosphatidylglycerol. The respiratory quinone was menaquinone-6. The genomic DNA G+C content was 43.6 mol%. Phylogenetic analysis based on 16S rRNA gene sequences and core genes showed that the novel isolate belonged to the genus and was most closely related to 42BKT (94.7 % sequence identity). The average nucleotide identity and digital DNA–DNA hybridization values between ST1-3 and 42BKT were 74.6 and 16.3 %, respectively. On the basis of the phenotypic, phylogenetic and genomic data presented here, strain ST1-3 represents a novel species of the genus , for which the name sp. nov. is proposed, with the type strain ST1-3 (=MCCC M25234=KCTC 25639).

  • Received:
  • Accepted:
  • Published Online:
Keyword(s): Epsilonproteobacteria , hydrogen-oxidizing and Sulfurovum mangrovi
Funding
This study was supported by the:
  • Scientific Research Foundation of Third Institute of Oceanography (Award No. 2019021)
    • Principle Award Recipient: ZongzeShao
  • China Postdoctoral Science Foundation (Award No. 2021TQ0397)
    • Principle Award Recipient: ZongzeShao
  • China Ocean Mineral Resources Research and Development Association (Award No. DY135-B2-01)
    • Principle Award Recipient: ZongzeShao
  • National Natural Science Foundation of China (Award No. 42176134)
    • Principle Award Recipient: ZongzeShao
© 2023 The Authors
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2023-11-02
2024-05-08
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Sulfurovum mangrovi sp. nov., an obligately chemolithoautotrophic, hydrogen-oxidizing bacterium isolated from coastal marine sediments
Int J Syst Evol Microbiol 73, 006142 (2023); https://doi.org/10.1099/ijsem.0.006142
/content/journal/ijsem/10.1099/ijsem.0.006142
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