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Abstract

Four Gram-positive, rod-shaped, none-sporeforming, non-motile isolates were obtained from various raw milk samples taken from the cooling tank on a research farm in Königswinter, Germany. Based on phylogenetic analysis of the 16S rRNA genes and whole genome sequences, all isolates were assigned to the genus , but were divided in two different groups. All isolates contained C 9 and C as predominant fatty acids, as well as traces of C. They all contained menaquinones MK-8 (H) and MK-9 (H) and produced mycolic acids characteristic for the majority of species belonging to the genus . 16S rRNA gene sequence similarity values to the closest related type strains DSM 45392 and DSM 20521 were below 98.7 %, average nucleotide identity values were below 86 % and digital DNA–DNA-hybridization values were below 25 %, indicating that the isolates represent two novel species. The names sp. nov. and sp. nov. are proposed, represented by the type strains LM112 (=DSM 116216=HAMBI 3782) and R4 (=DSM 116183=HAMBI 3785), respectively.

Keyword(s): Corynebacterium , dairy farm and raw milk
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2023-10-27
2024-12-09
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