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INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY logo

Volume 73, Issue 11

sp. nov. (formerly genomic species 3) and sp. nov. (formerly genomic species 8) isolated from female urinary microbiome No Access

Abstract

During an ongoing female urinary microbiome research study, strains c17Ua_112 and c31Ua_26 isolated from urine samples of a patient diagnosed with overactive bladder and a healthy postmenopausal woman, respectively, could not be allocated to any species with valid names. In this work, we aimed to characterize these strains. The 16S rRNA gene sequences confirmed that these strains are members of the genus . Phylogenetic analysis based on strongly supported two clades, one encompassing c17Ua_112 and nine other strains from the public database, and the other including c31Ua_26 and three other strains, which were distinct from currently recognized species of the genus . Likewise, the phylogenomic tree also showed that strains c17Ua_112 and c31Ua_26 formed independent and robust clusters. Average nucleotide identity (ANI) and digital DNA–DNA hybridization (dDDH) values between c17Ua_112 and c31Ua_26 were 79.27 and 27.4 %, respectively. Strain c17Ua_112 showed the highest ANI (94.8 %) and dDDH values (59.8 %) with UGent 18.01, and strain c31Ua_26 revealed highest ANI (84.2 %) and dDDH (29.1 %) values with GS 9838-1. Based on the data presented here, the two strains c17Ua_112 and c31Ua_26 represent two novel species of the genus , for which the names (c17Ua_112=DSM 113414=CCP 71) and (c31Ua_26=DSM 113415=CCP 72) are proposed.

  • Received:
  • Accepted:
  • Published Online:
Keyword(s): Gardnerella greenwoodii sp. nov , Gardnerella pickettii sp. nov. , Gardnerella vaginalis and whole genome sequencing
Funding
This study was supported by the:
  • Fundação para a Ciência e a Tecnologia (Award NORTH-01-0145-FEDER-000024)
    • Principle Award Recipient: SvetlanaUgarcina Perovic
  • Applied Molecular Biosciences Unit (Award UIDP/QUI/04378/2020)
    • Principle Award Recipient: TeresaGonçalves Ribeiro
  • Fundação para a Ciência e a Tecnologia (Award DL57/2016/CP1346/CT0034)
    • Principle Award Recipient: FilipaGrosso
  • Fundação para a Ciência e a Tecnologia (Award SFRH/BD/132497/2017)
    • Principle Award Recipient: MagdalenaKsiezarek
  • Fundação para a Ciência e a Tecnologia (Award SFRH/BD/05038/2020)
    • Principle Award Recipient: MárciaSousa
  • Fundação para a Ciência e a Tecnologia (Award LA/P/0140/2020)
    • Principle Award Recipient: NotApplicable
  • Fundação para a Ciência e a Tecnologia (Award UIDB/04378/2020)
    • Principle Award Recipient: NotApplicable
  • Fundação para a Ciência e a Tecnologia (Award UIDP/04378/2020)
    • Principle Award Recipient: NotApplicable
© 2023 The Authors
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2023-11-03
2024-05-08
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Gardnerella pickettii sp. nov. (formerly Gardnerella genomic species 3) and Gardnerella greenwoodii sp. nov. (formerly Gardnerella genomic species 8) isolated from female urinary microbiome
Int J Syst Evol Microbiol 73, 006140 (2023); https://doi.org/10.1099/ijsem.0.006140
/content/journal/ijsem/10.1099/ijsem.0.006140
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