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Volume 73, Issue 11

sp. nov., isolated from the phosphonate-amended seawater of the northwestern Pacific Ocean No Access

Abstract

A novel Gram-stain-negative, aerobic, rod-shaped bacterium, designated as HL-NP1, was isolated from the surface water of the northwestern Pacific Ocean after enrichment cultivation using the organic phosphorous compound of 2-aminoethylphosphonate. Phylogenetic analysis based on 16S rRNA gene sequences showed that the strain belonged to the genus , with the highest similarity to 40Bstr34 (98.7 %). The complete genome sequence of strain HL-NP1 comprised a circular chromosome of 5.58 Mbp and two circular plasmids of 0.15 and 0.22 Mbp. Comparison of the genome sequences between strains HL-NP1 and 40Bstr34 revealed that average nucleotide identity, average amino acid identity and digital DNA–DNA hybridization values (88.0, 86.4 and 33.9 %, respectively) were below the recommended cut-off levels for delineating bacterial species. Strain HL-NP1 showed optimal growth at 30 °C, pH 6.5–7.0, with 2.0–2.5 % (w/v) NaCl. The sole respiratory quinone was ubiquinone-10. The predominant fatty acid was summed feature 8 (C 6 and/or C 7). The polar lipids comprised diphosphatidylglycerol, phosphatidylcholine, phosphatidylethanolamine, phosphatidylglycerol, phosphatidylmonomethylethanolamine, an unidentified aminolipid and four unidentified lipids. The G+C content of the genomic DNA was 65.1 %. Based on phylogenetic, genotypic, phenotypic and chemotaxonomic data, strain HL-NP1 is proposed to represent a novel species of the genus , for which the name sp. nov. is proposed. The type strain is HL-NP1 (= KCCM 90499 = JCM 35838).

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  • Accepted:
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Keyword(s): Jiella pelagia , new species , northwestern Pacific Ocean and phosphonate
Funding
This study was supported by the:
  • National Research Foundation (Award NRF-2021M3I6A1091272)
    • Principle Award Recipient: ChungYeon Hwang
  • Korea Institute of Marine Science and Technology promotion (Award 20210427)
    • Principle Award Recipient: ChungYeon Hwang
© 2023 The Authors
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2023-11-02
2024-10-11
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Jiella pelagia sp. nov., isolated from the phosphonate-amended seawater of the northwestern Pacific Ocean
Int J Syst Evol Microbiol 73, 006139 (2023); https://doi.org/10.1099/ijsem.0.006139
/content/journal/ijsem/10.1099/ijsem.0.006139
/content/journal/ijsem/10.1099/ijsem.0.006139
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