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INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY logo

Volume 73, Issue 11

sp. nov., sp. nov. and sp. nov., three actinobacteria isolated from Western Pacific Ocean sediment No Access

Abstract

Three Gram-stain-positive, non-motile, short rod-shaped, catalase-positive and oxidase-negative actinomycete strains (SOB44, SOB72 and SOB77) were isolated from a deep-sea sediment sample collected from the Western Pacific Ocean. Cells of the three strains showed optimum growth at 30 °C and pH 7.0. Strains SOB44, SOB72 and SOB77 could tolerate up to 10, 9 and 9 % (w/v) NaCl concentration and grow at pH 5.0–12.0, 5.0–11.0 and 5.0–11.0, respectively. Phylogenetic results based on 16S rRNA gene sequences showed that the three isolates belonged to the genus and were identified as representing three novel species based on 78.0–93.1 % average nucleotide identity and 21.3–50.0 % DNA–DNA hybridization values with closely related reference strains. Strains SOB44, SOB72 and SOB77 showed highest 16S rRNA gene sequence similarity to CL-Z59 (99.2 %), SC8A-24 (99.2 %) and zg-579 (98.5 %), respectively. All three strains had MK-8(H) as the respiratory quinone, iso-C as the major fatty acid, and phosphatidylglycerol, diphosphatidylglycerol and phosphatidylinositol as the major polar lipids. The diagnostic diamino acid in the cell-wall peptidoglycan of all three isolates was -diaminopimelic acid. The DNA G+C contents of strains SOB44, SOB72 and SOB77 were 71.1, 72.9 and 72.9 mol%, respectively. Based on the phenotypic, phylogenetic and genotypic data, strains SOB44, SOB72 and SOB77 clearly represent three novel taxa within the genus , for which the names sp. nov. (type strain SOB44=JCM 35774= MCCC M28400), sp. nov. (type strain SOB72=JCM 35775=MCCC M28318) and sp. nov. (type strain SOB77=JCM 35776=MCCC M28544) are proposed.

  • Received:
  • Accepted:
  • Published Online:
Keyword(s): actinomycetes , Actinomycetota , Nocardioides , novel species , polyphasic taxonomy , sediment and Western Pacific Ocean
Funding
This study was supported by the:
  • Scientific Research Foundation of Third Institute of Oceanography, MNR (Award NO. 2019011)
    • Principle Award Recipient: GaiyunZhang
  • China Ocean Mineral Resources R&D Association (COMRA) Program (Award No. DY135-B2-01)
    • Principle Award Recipient: GaiyunZhang
  • National Microbial Resource Center (Award No. NMRC-2023-9)
    • Principle Award Recipient: GaiyunZhang
© 2023 The Authors
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/content/journal/ijsem/10.1099/ijsem.0.006138
2023-11-02
2024-05-09
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Nocardioides cremeus sp. nov., Nocardioides abyssi sp. nov. and Nocardioides oceani sp. nov., three actinobacteria isolated from Western Pacific Ocean sediment
Int J Syst Evol Microbiol 73, 006138 (2023); https://doi.org/10.1099/ijsem.0.006138
/content/journal/ijsem/10.1099/ijsem.0.006138
/content/journal/ijsem/10.1099/ijsem.0.006138
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