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Volume 73, Issue 11

sp. nov., a novel antibiotic-producing member of the genus Open Access

Abstract

Strain TÜ4103 was originally sampled from Java, Indonesia and deposited in the Tübingen strain collection under the name ‘ sp.’. The strain was found to be an antibiotic producer as strain TÜ4103 showed bioactivity against Gram-positive bacteria, such as and in bioassays. Strain TÜ4103 showed 16S rRNA gene sequence similarity of 99.65 % to DSM 101999 and 98.82 % to DSM 44781 and DSM 44780. Genome-based phylogenetic analysis revealed that strain TÜ4103 is closely related to DSM 44780 and DSM 44781. The digital DNA–DNA hybridization values between the genome sequences of strain TÜ4103 and its closest phylogenomic relatives, strains DSM 44780 and DSM 44781, were 43.0 and 42.9 %, respectively. Average nucleotide identity (ANI) values support this claim, with the highest ANI score of 91.14 % between TÜ4103 and being closely followed by an ANI value of 91.10 % between and TÜ4103. The genome of TÜ4103 has a size of 7.91 Mb with a G+C content of 74.05 mol%. Whole-cell hydrolysates of strain TÜ4103 are rich in -diaminopimelic acid, and rhamnose, galactose and mannose are characteristic as whole-cell sugars. The phospholipid profile contains phosphatidylethanolamine, diphosphatidylglycerol and glycophospholipid. The predominant menaquinones (>93.5 %) are MK-9(H) and MK-9(H). Based on the phenotypic, genotypic and genomic characteristics, strain TÜ4103 (=DSM 114396=CECT 30712) merits recognition as the type strain of a novel species of the genus , for which the name sp. nov. is proposed.

  • Received:
  • Accepted:
  • Published Online:
Keyword(s): antibiotic producer , Kitasatospora , novel species , polyphasic taxonomy and rare actinomycete
Funding
This study was supported by the:
  • Deutsches Zentrum für Infektionsforschung (Award TTU 09.826)
    • Principle Award Recipient: YvonneMast
  • Leibniz-Gemeinschaft (Award K445/2022)
    • Principle Award Recipient: YvonneMast
© 2023 The Authors
  • This is an open-access article distributed under the terms of the Creative Commons Attribution License. This article was made open access via a Publish and Read agreement between the Microbiology Society and the corresponding author’s institution.
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2023-11-02
2024-05-08
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Kitasatospora fiedleri sp. nov., a novel antibiotic-producing member of the genus Kitasatospora
Int J Syst Evol Microbiol 73, 006137 (2023); https://doi.org/10.1099/ijsem.0.006137
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