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Abstract

A Gram-stain-negative, aerobic, rod-shaped, non-motile and non-flagellated novel bacterial strain, designated MAH-24, was isolated from the rhizospheric soil of a pine garden. The colonies were observed to be orange-coloured, smooth, spherical and 0.4–0.8 mm in diameter when grown on Reasoner's 2A agar medium for 2 days. Strain MAH-24 was found to be able to grow at 10–35 °C, at pH 6.0–9.0 and in the presence of 0–1.0 % NaCl (w/v). The strain was found to be positive for the catalase and oxidase tests. The strain was positive for hydrolysis of aesculin and -tyrosine. According to the 16S rRNA gene sequence comparisons, the isolate was identified as a member of the genus and to be closely related to MAH-26 (99.2 % sequence similarity). The novel strain MAH-24 has a draft genome size of 5 918 133 bp (13 contigs), annotated with 4613 protein-coding genes, 47 tRNA and three rRNA genes. The average nucleotide identity (ANI) and digital DNA–DNA hybridization (dDDH) values between strain MAH-24 and the closest type strain MAH-26 were in the range of 85.3 and 29.9 %, respectively. genome mining revealed that both novel strain MAH-24 and MAH-26 have a significant potential for the production of novel natural products in the future. The genomic DNA G+C content was determined to be 41.0 mol%. The predominant isoprenoid quinone was menaquinone-7. The major fatty acids were identified as C iso, C iso G and C iso 3OH. On the basis of dDDH, ANI, genotypic, chemotaxonomic and physiological data, strain MAH-24 represents a novel species within the genus , for which the name sp. nov. is proposed, with MAH-24 (=KACC 19747=CGMCC 1.13659) as the type strain.

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2023-11-08
2024-10-03
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