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INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY logo

Volume 73, Issue 11

Isolation and characterization of sp. nov., and genome mining of for biosynthetic gene cluster prediction No Access

Abstract

A Gram-stain-negative, aerobic, rod-shaped, non-motile and non-flagellated novel bacterial strain, designated MAH-24, was isolated from the rhizospheric soil of a pine garden. The colonies were observed to be orange-coloured, smooth, spherical and 0.4–0.8 mm in diameter when grown on Reasoner's 2A agar medium for 2 days. Strain MAH-24 was found to be able to grow at 10–35 °C, at pH 6.0–9.0 and in the presence of 0–1.0 % NaCl (w/v). The strain was found to be positive for the catalase and oxidase tests. The strain was positive for hydrolysis of aesculin and -tyrosine. According to the 16S rRNA gene sequence comparisons, the isolate was identified as a member of the genus and to be closely related to MAH-26 (99.2 % sequence similarity). The novel strain MAH-24 has a draft genome size of 5 918 133 bp (13 contigs), annotated with 4613 protein-coding genes, 47 tRNA and three rRNA genes. The average nucleotide identity (ANI) and digital DNA–DNA hybridization (dDDH) values between strain MAH-24 and the closest type strain MAH-26 were in the range of 85.3 and 29.9 %, respectively. genome mining revealed that both novel strain MAH-24 and MAH-26 have a significant potential for the production of novel natural products in the future. The genomic DNA G+C content was determined to be 41.0 mol%. The predominant isoprenoid quinone was menaquinone-7. The major fatty acids were identified as C iso, C iso G and C iso 3OH. On the basis of dDDH, ANI, genotypic, chemotaxonomic and physiological data, strain MAH-24 represents a novel species within the genus , for which the name sp. nov. is proposed, with MAH-24 (=KACC 19747=CGMCC 1.13659) as the type strain.

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Keyword(s): digital DNA–DNA hybridization , genome sequence , in silico genome mining , Pinibacter soli and secondary metabolites
© 2023 The Authors
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2023-11-08
2024-05-08
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Isolation and characterization of Pinibacter soli sp. nov., and in silico genome mining of Pinibacter for biosynthetic gene cluster prediction
Int J Syst Evol Microbiol 73, 006136 (2023); https://doi.org/10.1099/ijsem.0.006136
/content/journal/ijsem/10.1099/ijsem.0.006136
/content/journal/ijsem/10.1099/ijsem.0.006136
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