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Volume 73, Issue 10

sp. nov., isolated from the intestine of the deep-sea loosejaw dragonfish, Open Access

Abstract

A novel bacterial strain, APC 3343, was isolated from the intestine of a deep-sea loosejaw dragon fish, , caught at a depth of 1000 m in the Northwest Atlantic Ocean. Cells were aerobic, rod-shaped, yellow/orange-pigmented, non-motile and Gram-negative. Growth of strain APC 3343 was observed at 4–30 °C (optimum, 21–25 °C), pH 5.5–10 (optimum, pH 7–8) and 0.5–8 % (w/v) NaCl (optimum, 2–4 %). Phylogenetic analysis based on 16S rRNA gene sequences showed that strain APC 3343 was most closely related to members of the genus , with the most closely related type strains being Kr9-9 (98.46 % identity), F081-2 (98.07 %), CECT 7946 (97.93 %), KMM 6491 (97.79 %) and HL2-2 (97.79 %). Major fatty acids (>10 % of total) were iso-C 3-OH, iso-C, anteiso-C and iso-C 3-OH. The predominant respiratory quinone was menaquinone-6 (MK-6). Polar lipids were phosphatidylethanolamine, three unknown aminolipids and eight unknown lipids. The draft genome sequence was 3.8 Mb in length with a G+C content of 33.43 mol%. Based on the phenotypic characteristics and phylogenetic analysis, strain APC 3343 is deemed to be a novel species of the genus , and for which the name sp. nov. is proposed. The type strain of this species is APC 3343 (=DSM 115832=NCIMB 15464).

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  • Accepted:
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Keyword(s): deep sea , fish isolate , gut microbiome , sp. nov. and Winogradskyella
Funding
This study was supported by the:
  • European Research Council (Award 101054719)
    • Principle Award Recipient: PaulRoss
  • Science Foundation Ireland (Award SFI/12/RC/2273_P2)
    • Principle Award Recipient: PaulRoss
© 2023 The Authors
  • This is an open-access article distributed under the terms of the Creative Commons Attribution License. This article was made open access via a Publish and Read agreement between the Microbiology Society and the corresponding author’s institution.
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2023-10-25
2024-05-08
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Winogradskyella bathintestinalis sp. nov., isolated from the intestine of the deep-sea loosejaw dragonfish, Malacosteus niger
Int J Syst Evol Microbiol 73, 006135 (2023); https://doi.org/10.1099/ijsem.0.006135
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