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INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY logo

Volume 73, Issue 10

gen. nov., sp. nov., the first characterized representative of the candidate family env.OPS 17 and proposal of fam. nov. Open Access

Abstract

16S rRNA sequence types associated with the candidate family env.OPS 17 have been reported from various environments, but no representatives have been characterized and validly named. Bacteria of env.OPS 17 are affiliated with the order and were first detected more than two decades ago in the vicinity of a thermal spring in Yellowstone National Park. Strain Swamp196, isolated from the soil surrounding a swamp in Northern Germany, is the first characterized representative of candidate family env.OPS 17. Cells of strain Swamp196 are rod-shaped, non-motile, non-spore-forming, non-capsulated and stain Gram-negative. Colonies are small and orange-coloured. The strain is mesophilic and grows under aerobic or microaerophilic conditions. It grows chemo-organotrophically over a narrow range of pH and exclusively on proteinaceous substrates. The major cellular fatty acids are iso-C, iso-C 10, C 9 and C 7 and the major polar lipids are two unidentified aminophospholipids, one unidentified aminolipid and one unidentified lipid. The predominant respiratory quinone is MK-7. The DNA G+C content of genomic DNA is 35.5 mol%. Strain Swamp196 is related to AR-3-17, Hh36 and Dae 13 with 16S rRNA gene sequence similarity of 84.1, 83.8 and 83.5 %, respectively. Based on our phenotypic, genomic and phylogenetic analysis, we propose the novel species sp. nov (type strain Swamp196=DSM 105849=CECT 30420) of the novel genus gen. nov. and the novel family fam. nov.

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  • Accepted:
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Keyword(s): Aurantibacillus , env.OPS 17 , narrow pH range and soil bacteria
Funding
This study was supported by the:
  • Deutsche Forschungsgemeinschaft (Award OV 20/21-1)
    • Principle Award Recipient: JörgOvermann
© 2023 The Authors
  • This is an open-access article distributed under the terms of the Creative Commons Attribution License. This article was made open access via a Publish and Read agreement between the Microbiology Society and the corresponding author’s institution.
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2023-10-27
2024-05-09
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Aurantibacillus circumpalustris gen. nov., sp. nov., the first characterized representative of the Bacteroidota candidate family env.OPS 17 and proposal of Aurantibacillaceae fam. nov.
Int J Syst Evol Microbiol 73, 006134 (2023); https://doi.org/10.1099/ijsem.0.006134
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