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Volume 73, Issue 11

sp. nov. and sp. nov., isolated from coastal sediment, and genome analysis and habitat distribution of the genus No Access

Abstract

Three strains, X102, S1101 and S6413, were isolated from sediment samples from different coasts of Weihai, PR China. All strains were Gram-stain-negative, rod-shaped and non-motile. The predominant fatty acids of all strains were iso-C and summed feature 3 (C 7/C 6) and the major polar lipids comprised phosphatidylethanolamine, phosphatidylglycerol and diphosphatidylglycerol. Strains X102 and S1101 shared 100 % 16S rRNA gene sequence similarity, and strains S1101/X102 and S6413 had 95.4 % similarity. The average nucleotide identity (ANI) and digital DNA–DNA hybridization (dDDH) values between strains S1101 and X102 were 99.9 and 99.2 %, respectively. Strain S1101 had ANI values of 69.1–72.9% and dDDH values of 17.9–20.5 % to members of the genus . Strain S6413 had ANI values of 69.1–77.5% and dDDH values of 17.6–21.5 % to members of the genus . The results of phylogenetic and comparative genomic analysis showed that the three strains belong to two novel species in the genus , and strains X102 and S1101 represented one novel species, and strain S6413 represented another novel species. The result of BOX-PCR and genomic analysis showed that X102 and S1101 were not the same strain. The phylogenetic analyses and genomic comparisons, combined with phylogenetic, phenotypic and chemotaxonomic features, strongly supported that the three strains should be classified as representing two novel species of the genus , for which the names sp. nov. and sp. nov. are proposed, respectively. The type strains of the two novel species are S1101 (=KCTC 92642=MCCC 1H01359) and S6413 (=KCTC 92641=MCCC 1H01362), respectively. In addition, all previously described isolates of were isolated from marine environments, but our study showed that is also distributed in non-/low-saline habitats (e.g. animal gut, soil and indoor surface), which broadened our perception of the environmental distribution of .

  • Received:
  • Accepted:
  • Published Online:
Keyword(s): genome analysis , habitat distribution , Marinicella gelatinilytica sp. nov. , Marinicella marina sp. nov. , novel species and polyphasic approach
Funding
This study was supported by the:
  • Natural Science Foundation of Shandong Province, China (Award ZR2023QD187)
    • Principle Award Recipient: JingZhang
  • Postdoctoral Science Foundation of Jiangsu Province (Award 2022M721923)
    • Principle Award Recipient: JingZhang
  • National Natural Science Foundation of China (Award 41876166)
    • Principle Award Recipient: Zong-JunDu
  • Science & Technology Fundamental Resources Investigation Program (Award 2019FY100700)
    • Principle Award Recipient: Zong-JunDu
  • Science & Technology Fundamental Resources Investigation Program (Award 2022FY101100)
    • Principle Award Recipient: Zong-JunDu
© 2023 The Authors
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2024-05-08
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Marinicella marina sp. nov. and Marinicella gelatinilytica sp. nov., isolated from coastal sediment, and genome analysis and habitat distribution of the genus Marinicella
Int J Syst Evol Microbiol 73, 006130 (2023); https://doi.org/10.1099/ijsem.0.006130
/content/journal/ijsem/10.1099/ijsem.0.006130
/content/journal/ijsem/10.1099/ijsem.0.006130
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