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INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY logo

Volume 73, Issue 11

sp. nov., isolated from the soil of a forest No Access

Abstract

A Gram-negative, rod-shaped, aerobic and light pink-pigmented bacterium, designated XAAS-A31, was isolated from the soil of a forest located near Hotan City, Xinjiang, PR China. Polyphasic, taxonomic and phylogenomic analyses were used to determine the taxonomy position of the strain. Phylogenetic analysis based on 16S rRNA gene sequence analysis indicated that XAAS-A31 belongs to the genus , family , and shows highest sequence similarity to XAAS-R86 (96.2 %). The digital DNA–DNA hybridization (22.0 %–19.2 %) and orthologous average nucleotide identity (74.1 %–72.7 %) values relative to closest validly published species were lower than the recommended thresholds of 70 and 96 %, respectively. The cells grew at 4–40 °C (optimum, 28–30 °C), at pH 6.5–8.5 (pH 7.0–7.5) and with 0–8% NaCl (0.5–2.0% NaCl). The main respiratory quinone of XAAS-A31 is MK-7, and the principal cellular fatty acids are iso-C, iso-C 3OH and summed feature 4 (iso-C I and/or anteiso-C B). The major polar lipids are phosphatidylethanolamine, three unidentified amino-phospholipids, one unidentified glycolipid and eight unidentified lipids. The genome length of strain XAAS-A31 is 5.48 Mbp with a DNA G+C content of 44.2 mol% and 4013 protein-coding genes. Phenotypic and genotypic data suggested that XAAS-A31 represents a novel species, for which we propose the name sp. nov. and type strain XAAS-A31 (=CCTCC AB 2017162=KCTC 62049).

  • Received:
  • Accepted:
  • Published Online:
Keyword(s): Hymenobacteraceae , Pontibacter , Populus euphratica and soil
Funding
This study was supported by the:
  • Project of Renovation Capacity Building for the Young Sci-Tech Talents Sponsored by Xinjiang Academy of Agricultural Sciences (Award xjnkq-2020019)
    • Principle Award Recipient: GhenijanOsman
© 2023 The Authors
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2023-11-02
2024-05-08
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Pontibacter harenae sp. nov., isolated from the soil of a Populus euphratica forest
Int J Syst Evol Microbiol 73, 006128 (2023); https://doi.org/10.1099/ijsem.0.006128
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