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Volume 73, Issue 11

sp. nov., a moderately halophilic bacterium isolated from an oilfield Open Access

Abstract

A novel alkaliphilic, Gram-stain-positive, moderately halophilic, rod-shaped, endospore-forming, motile, facultatively anaerobic bacterium (DQ-9) was isolated from a sediment sample collected from Daqing oilfield in China, and characterized by a polyphasic taxonomic approach. Strain DQ-9 formed yellow pigment and grew occurred at salinities of 1–12 % (w/v) NaCl (optimum, 8 %) and at 10–40 °C (optimum, 30–35 °C), at pH 7.5–10.5 (optimum, pH 9.0–9.5). It was catalase-positive, but oxidase-negative. Based on the analysis of 16S rRNA gene sequences, DQ-9 was classified into the genus and exhibited the highest similarities (98.37 %) to JSM 071004. Digital DNA–DNA hybridization and average nucleotide identity values between strain DQ-9 and the most closely related strain, DSM 19794, were determined to be 72.0 and 21.6 %, respectively. The polar lipids were constituted by diphosphatidylglycerol, phosphatidylglycerol and phosphatidylethanolamine. The major fatty acids (>5 %) comprised anteiso-C, anteiso-C, iso-C, iso-C and C. The cell-wall peptidoglycan contained -diaminopimelic acid, and menaquinone-7 was identified as the primary respiratory quinone. The DNA G+C content was 37.5 mol%. Through chemotaxonomic, physiological, and biochemical characterization, strain DQ-9 could be clearly distinguished from the closest species. Based on provided data, strain DQ-9 is proposed to represent a novel species, sp. nov., within the genus . The type strain is DQ-9 (=ACCC 60415=KCTC 33936).

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  • Accepted:
  • Published Online:
Keyword(s): alkaliphile , moderately halophilic , oil field , Salipaludibacillus and Salipaludibacillus daqingensis sp. nov.
Funding
This study was supported by the:
  • Guangzhou Science and Technology Innovation Center (Award CAAS-ZDRW202201)
    • Principle Award Recipient: XiaoxiaZhang
  • Special Funds for the Basic Research and Development Program in the Central Non-profit Research Institutesof China (Award 1610042023004)
    • Principle Award Recipient: HaishengWang
© 2023 The Authors
  • This is an open-access article distributed under the terms of the Creative Commons Attribution License. This article was made open access via a Publish and Read agreement between the Microbiology Society and the corresponding author’s institution.
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2023-11-02
2024-05-08
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Salipaludibacillus daqingensis sp. nov., a moderately halophilic bacterium isolated from an oilfield
Int J Syst Evol Microbiol 73, 006126 (2023); https://doi.org/10.1099/ijsem.0.006126
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