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Volume 73, Issue 11

sp. nov., an asexual yeast species isolated from insect frass No Access

Abstract

Two strains (NYNU 218101 and NYNU 218104) of an asexual yeast species were isolated from insect frass collected in insect tunnels of red leaf plum trees in the Henan Province, central China. Molecular phylogenetic analysis of the D1/D2 domain of the large subunit rRNA gene and the internal transcribed spacer (ITS) region showed that these two strains belonged to the genus , with as the closest known species. They differed from the type strain of by 0.6 % substitutions (three substitutions and one gap) in the D1/D2 domain and by 5.1 % substitutions (19 substitutions and six gaps) in the ITS region, respectively. When compared with the partial , and gene sequences, they differed by 3 % (26 substitutions), 2.7 % (25 substitutions) and 9 %(54 substitutions) from NRRL YB-3246 in these regions. Physiologically, they also differed from its closest known species based on the ability to assimilate inulin and galactitol, as well as to grow in 0.1 % cycloheximide and its inability to ferment maltose and raffinose. In order to classify the two new isolates based on morphological and molecular evidence, we proposed the description of a novel species sp. nov. with strain JCM 35735 as holotype (Mycobank MB 849101).

  • Received:
  • Accepted:
  • Published Online:
Keyword(s): Danielozyma pruni , insect frass and yeast species
Funding
This study was supported by the:
  • National Natural Science Foundation of China (Award 31570021)
    • Principle Award Recipient: Feng-LiHui
© 2023 The Authors
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/content/journal/ijsem/10.1099/ijsem.0.006124
2023-11-22
2024-05-08
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Danielozyma pruni sp. nov., an asexual yeast species isolated from insect frass
Int J Syst Evol Microbiol 73, 006124 (2023); https://doi.org/10.1099/ijsem.0.006124
/content/journal/ijsem/10.1099/ijsem.0.006124
/content/journal/ijsem/10.1099/ijsem.0.006124
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