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INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY logo

Volume 73, Issue 11

sp. nov. and sp. nov., two novel species with high plant-promoting potential isolated from wetland plants in China No Access

Abstract

Two novel plant growth-promoting, rod-shaped, Gram-positive and non-motile rhizobacteria, W1N and W2R, were isolated from wetland plants and respectively, in China. The results of the 16S rRNA sequence alignment analysis showed that they were related to , with the highest similarity to (98.7 %) and (98.5 %) for strain W1N, and to (98.1 %) and (98.0 %) for strain W2R. Phylogenetic analyses based on 16S rRNA gene sequences and 92 conserved concatenated proteins suggested that the two strains belong to the genus and were placed in two separate novel phylogenetic clades. The genome sizes of the two strains were 3.2 and 3.7 Mb, and the G+C contents were 71.7 and 68.5 mol%, respectively. The comparative genome results showed that the average nucleotide identity values between W1N and W2R and other species ranged from 73.5 to 83.6 %, and the digital DNA–DNA hybridization values ranged from 19.7 to 26.8 %. These two strains show physiological and biochemical features that differ from those of closely related species. Rhamnose, galactose and glucose were present in the characteristic sugar fractions of strains W1N and W2R. The peptidoglycan of strains W1N and W2R contained the amino acids ornithine, alanine and aspartic acid. C anteiso, C anteiso and C iso were the predominant cellular fatty acids in W1N and W2R. Phosphatidylglycerol and diphosphatidylglycerol are major polar lipid components. Strain W1N not only formed bacterial biofilms but also had the ability to solubilize phosphorus and produce indole-3-acetic acid. Strain W2R had siderophore-producing and lignin-degrading properties. Based on their genetic and phenotypic characteristics, strains W1N and W2R were classified as novel bacteria in the genus and designated as sp. nov. (type strain W1N=ACCC 61807=GDMCC 1.2966=JCM 35339) and sp. nov. (type strain W2R=ACCC 61808=GDMCC 1.2967=JCM 35340).

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Keyword(s): Microbacterium , Microbacterium festucae , Microbacterium nymphoidis , plant-growth promoting and polyphasic taxonomy
Funding
This study was supported by the:
  • Central Non-profit Research Institution of Chinese Academy of Forestry (Award CAFYBB2020SZ006)
© 2023 The Authors
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2023-11-02
2024-05-08
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Microbacterium nymphoidis sp. nov. and Microbacterium festucae sp. nov., two novel species with high plant-promoting potential isolated from wetland plants in China
Int J Syst Evol Microbiol 73, 006121 (2023); https://doi.org/10.1099/ijsem.0.006121
/content/journal/ijsem/10.1099/ijsem.0.006121
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