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Volume 73, Issue 10

sp. nov., isolated from biochemical tank sludge No Access

Abstract

A Gram-stain-positive, coccoid-shaped, non-spore-forming, facultatively anaerobic bacterium, designated YN-L-12, was isolated from the activate sludge of a pesticide plant. Colonies on tryptone soya agar were small, white, opaque and circular. Phylogenetic analyses based on 16S rRNA gene sequences indicated that strain YN-L-12 belonged to the genus of , and showed the highest similarity to 1805-02 (97.0 %), followed by H21T32 (96.5 %), 1804-02 (95.6 %) and EX-07 (95.4 %). The strain grew at 15–37 °C (optimum, 30 °C), with 0–6.5 % (w/v) NaCl (optimum, 0.5 %) and at pH 7–9 (optimum, pH 7.5). The major fatty acids were C ω9, C ω9 and C. The major polar lipids were diphosphatidylglycerol, phosphatidylglycerol, phosphatidylethanolamine, glycolipid and an unidentified lipid. The DNA G+C content of the strain was 41.1 mol%. Average nucleotide identity values between strain YN-L-12 and 1805-02 and H21T32 were 72.8 and 72.3 %, respectively. The digital DNA–DNA hybridization values between YN-L-12 and 1805-02 and H21T32 were 24.1 and 20.3 %, respectively. According to the results of phenotypic, chemotaxonomic and phylogenetic analyses, strain YN-L-12 represents a novel species of the genus , for which the name sp. nov. is proposed, with strain YN-L-12 (=KCTC 43533=CCTCC AB 2022400) as the type strain.

  • Received:
  • Accepted:
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Keyword(s): 16S rRNA gene sequence , Jeotgalibaca caeni sp. nov. , sludge and taxonomy
Funding
This study was supported by the:
  • Special Fund for Scientific Innovation Strategy-Construction of High-level Academy of Agriculture Science (Award BM2022019)
    • Principle Award Recipient: NotApplicable
  • National Natural Science Foundation of China (Award No. 32170128)
    • Principle Award Recipient: NotApplicable
© 2023 The Authors
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2023-10-27
2024-05-08
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Jeotgalibaca caeni sp. nov., isolated from biochemical tank sludge
Int J Syst Evol Microbiol 73, 006116 (2023); https://doi.org/10.1099/ijsem.0.006116
/content/journal/ijsem/10.1099/ijsem.0.006116
/content/journal/ijsem/10.1099/ijsem.0.006116
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