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Volume 73, Issue 11

sp. nov., a biological control bacterium isolated from the common bean ( L.) crop in Sinaloa, Mexico No Access

Abstract

Strain FSQ1 was isolated from the rhizosphere of the common bean ( L.) crop sampled in a commercial field located in the Gabriel Leyva Solano community, which belongs to the Guasave municipality (state of Sinaloa, Mexico). Based on its full-length 16S rRNA gene sequence, strain FSQ1 was assigned to the genus (100 % similarity). This taxonomic affiliation was supported by its morphological and metabolic traits. Strain FSQ1 was a Gram-stain-positive bacterium with the following characteristics: rod-shaped cells, strictly aerobic, spore forming, catalase positive, reduced nitrate to nitrite, hydrolysed starch and casein, grew in the presence of lysozyme and 2 % NaCl, utilized citrate, grew at pH 6.0–8.0, produced acid from glucose, was unable to produce indoles from tryptophan, and presented biological control against . The whole-genome phylogenetic results showed that strain FSQ1 formed an individual clade in comparison with highly related species. In addition, the maximum values for average nucleotide identity and from Genome-to-Genome Distance Calculator analysis were 91.57 and 44.20 %, respectively, with TU-B-10. Analysis of its fatty acid content showed the ability of strain FSQ1 to produce fatty acids that are not present in closely related species, such as C and C. Thus, these results provide strong evidence that strain FSQ1 represents a novel species of the genus , for which the name sp. nov. is proposed. The type strain is FSQ1 (CM-CNRG TB51=LBPCV FSQ1).

  • Received:
  • Accepted:
  • Published Online:
Keyword(s): average nucleotide identity (ANI) , biological control agent , food security , Genome-to-Genome Distance Calculator (GGDC) and whole-genome phylogenetic
Funding
This study was supported by the:
  • PROFAPI-ITSON (Award PROFAPI 2023_0002)
    • Principle Award Recipient: Sergiode los Santos Villalobos
  • CONACYT (Award 257246)
    • Principle Award Recipient: Sergiode los Santos Villalobos
  • CONACYT (Award 1774)
    • Principle Award Recipient: Sergiode los Santos Villalobos
© 2023 The Authors
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2023-11-01
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Bacillus mexicanus sp. nov., a biological control bacterium isolated from the common bean (Phaseolus vulgaris L.) crop in Sinaloa, Mexico
Int J Syst Evol Microbiol 73, 006110 (2023); https://doi.org/10.1099/ijsem.0.006110
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