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Abstract

A Gram-stain-positive, rod-shaped, endospore-forming, aerobic bacterial strain, designated ZS111008, was isolated from high-temperature Daqu, a starter for production of Chinese Jiang-flavour Baijiu, and was characterized by polyphasic taxonomy. This novel isolate grew in the presence of 0–5 % (w/v) NaCl, at pH 6.0–9.0 and 25–45 °C; optimum growth was observed with 1 % (w/v) NaCl, at pH 8.0 and 30 °C. A comparative analysis of the 16S rRNA gene sequence (1461 bp) of strain ZS111008 showed highest similarity to DSM12223 (96.7%), followed by PN5 (96.6%) and AMCK01000046 (96.5%). The DNA G+C content of strain ZS111008 was 37.21 mol%. The respiratory quinone was identified as menaquinone-7 and the major polar lipids were diphosphatidylglycerol, phosphatidylglycerol, phosphatidylethanolamine, phosphatidylserine and one unknown phospholipid. Lys was detected as the diagnostic diamino acid in the cell wall. Based on morphological characteristics, chemotaxonomic characteristics and physiological properties, strain ZS111008 represents a novel species of the genus , for which the name sp. nov. is proposed. The type strain for this proposed species is ZS111008 (=CGMCC 1.19455=JCM 35214).

Funding
This study was supported by the:
  • National Science and Technology Fundamental Resources Investigation Program of China (Award 2021FY100900)
    • Principle Award Recipient: Zhen-MingLu
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2023-11-01
2024-11-11
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