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INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY logo

Volume 73, Issue 10

sp. nov., isolated from caecal contents of domestic pigs () Open Access

Abstract

During the 2021 European Food Safety Authority coordinated harmonized monitoring of antimicrobial resistance in species in Slovenia, five -like strains were cultured from caeca of a total of 104 domestic pigs that could not be identified using the standard-prescribed biochemical tests or MALDI-TOF MS. The isolates were obtained using the standard ISO 10272 procedure for the isolation of thermotolerant with prolonged cultivation time. Small -like colonies were observed on mCCDA and CASA agar plates after 2–4 days of incubation; dark-field microscopy revealed relatively big spirilli-shaped bacteria exhibiting characteristic -like motility. The cells were 1.5–3 µm long and 0.5–0.7 µm wide, Gram-negative, oxidase-positive and catalase-positive. MALDI-TOF mass spectra were distinctive and consistent, but with low MALDI-TOF MS log scores and the closest matches being those of and . All five strains underwent whole-genome sequencing. Analysis of 16S rRNA gene sequences revealed that the isolates were most similar (98.3–98.4 % identity) to . Pairwise average nucleotide identity (ANI) values revealed that the five studied strains shared pairwise ANI of 96.2–96.5 % but were clearly distinct from the previously described species (ANI ≤72.8 %). The core genome-based phylogeny confirmed that the new strains form a distinct and well-supported clade within the genus . The conducted polyphasic taxonomic analysis confirmed that the five strains represent a novel species for which the name sp. nov. is suggested, with strain 46386 (=DSM 115534=CCUG 76865) as the type strain.

  • Received:
  • Accepted:
  • Published Online:
Keyword(s): caecum , Campylobacter , domestic pig (Sus scrofa domesticus) and novel species
Funding
This study was supported by the:
  • Javna Agencija za Raziskovalno Dejavnost RS (Award P4-0092)
    • Principle Award Recipient: NotApplicable
© 2023 The Authors
  • This is an open-access article distributed under the terms of the Creative Commons Attribution License.
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2023-10-25
2024-05-08
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Campylobacter magnus sp. nov., isolated from caecal contents of domestic pigs (Sus scrofa domesticus)
Int J Syst Evol Microbiol 73, 006108 (2023); https://doi.org/10.1099/ijsem.0.006108
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