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Volume 73, Issue 11

sp. nov., isolated from contaminated freshwater sediment No Access

Abstract

Two Gram-stain-negative bacterial strains, S13-6-6 and S13-6-22, were isolated from sediment sample collected at a water depth of 4 m from Lake Hongze, Jiangsu Province, PR China. The cells of strains S13-6-6 and S13-6-22 were non-spore-forming, aerobic, non-motile and formed orange colonies on R2A agar. Comparative 16S rRNA gene sequence studies revealed a clear affiliation of the two strains with he phylum , and revealed the highest pairwise sequence similarities with H32-4 (97.8 %), NJ-8 (97.8 %), TTM-7 (97.4 %) and SS2-56 (97.4 %). The results of phylogenetic analysis based on 16S rRNA gene sequences indicated that the strains formed a clear phylogenetic lineage with the genus . The major fatty acids were identified as iso-CG, iso-C, iso-C 3-OH and summed feature 3 (Cω7 and/or Cω6) (>10 %), and the respiratory quinone was identified as menaquinone MK-7. The polar lipids consisted of phosphatidylethanolamine, two unidentified aminolipids, an unidentified phospholipid and six unidentified lipids. The genomic DNA G+C content was determined to be 40.2 mol% (HPLC) for strain S13-6-6 and 40.3 % (genome) for strain S13-6-22. The combined genotypic and phenotypic data indicated that strains S13-6-6 and S13-6-22 represent a novel species of the genus , for which the name sp. nov. is proposed. The type strain is S13-6-22 (=CGMCC 1.17450 =JCM 35802).

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Keyword(s): contaminated sediment , Lacibacter , Lacibacter sediminis , Lake Hongze , polyphasic taxonomy and strain S13-6-22
© 2023 The Authors
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2023-11-01
2024-05-08
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Lacibacter sediminis sp. nov., isolated from contaminated freshwater sediment
Int J Syst Evol Microbiol 73, 006105 (2023); https://doi.org/10.1099/ijsem.0.006105
/content/journal/ijsem/10.1099/ijsem.0.006105
/content/journal/ijsem/10.1099/ijsem.0.006105
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