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Abstract

Two Gram-stain-negative bacterial strains, S13-6-6 and S13-6-22, were isolated from sediment sample collected at a water depth of 4 m from Lake Hongze, Jiangsu Province, PR China. The cells of strains S13-6-6 and S13-6-22 were non-spore-forming, aerobic, non-motile and formed orange colonies on R2A agar. Comparative 16S rRNA gene sequence studies revealed a clear affiliation of the two strains with he phylum , and revealed the highest pairwise sequence similarities with H32-4 (97.8 %), NJ-8 (97.8 %), TTM-7 (97.4 %) and SS2-56 (97.4 %). The results of phylogenetic analysis based on 16S rRNA gene sequences indicated that the strains formed a clear phylogenetic lineage with the genus . The major fatty acids were identified as iso-CG, iso-C, iso-C 3-OH and summed feature 3 (Cω7 and/or Cω6) (>10 %), and the respiratory quinone was identified as menaquinone MK-7. The polar lipids consisted of phosphatidylethanolamine, two unidentified aminolipids, an unidentified phospholipid and six unidentified lipids. The genomic DNA G+C content was determined to be 40.2 mol% (HPLC) for strain S13-6-6 and 40.3 % (genome) for strain S13-6-22. The combined genotypic and phenotypic data indicated that strains S13-6-6 and S13-6-22 represent a novel species of the genus , for which the name sp. nov. is proposed. The type strain is S13-6-22 (=CGMCC 1.17450 =JCM 35802).

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2023-11-01
2024-06-15
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